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Assay systems for the evaluation of carcinogen interaction with human tissues are essential for assessing cancer risk. Human hair roots (HHR) are a readily obtainable epithelial tissue source that have been employed for investigating inherited enzyme activities. In this study HHR were found to possess cytochrome P-450-dependent 7-ethoxyresorufin-O-deethylase (ERD) activity which measures cytochrome P-450 isoenzymes that are highly specific (in the order of greater than 95%) markers for the metabolic activation of many environmental carcinogenic substances such as the polycyclic aromatic hydrocarbons (PAHs). Topical application of PAHs (in liquor carbonis detergens) to the scalp of human volunteers was found to enhance the activity of this enzyme in freshly plucked hair roots. Oral and topical administration of ketoconazole to the same subjects resulted in an appreciable (up to 73%) inhibition of detectable enzyme activity. Our data suggest that measurement of ERD in HHR may be a useful marker for the study of toxifying species of cytochrome P-450 isozymes in human populations.
H F Merk, and
H Mukhtar, and
B Schutte, and
I Kaufmann, and
M Das, and
D R Bickers
July 1984,
Analytical biochemistry,
H F Merk, and
H Mukhtar, and
B Schutte, and
I Kaufmann, and
M Das, and
D R Bickers
January 1987,
Developmental pharmacology and therapeutics,
H F Merk, and
H Mukhtar, and
B Schutte, and
I Kaufmann, and
M Das, and
D R Bickers
January 1992,
Toxicology in vitro : an international journal published in association with BIBRA,
H F Merk, and
H Mukhtar, and
B Schutte, and
I Kaufmann, and
M Das, and
D R Bickers
August 1994,
European journal of pharmacology,
H F Merk, and
H Mukhtar, and
B Schutte, and
I Kaufmann, and
M Das, and
D R Bickers
June 1988,
Biokhimiia (Moscow, Russia),
H F Merk, and
H Mukhtar, and
B Schutte, and
I Kaufmann, and
M Das, and
D R Bickers
December 1994,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
H F Merk, and
H Mukhtar, and
B Schutte, and
I Kaufmann, and
M Das, and
D R Bickers
August 2017,
Aquatic toxicology (Amsterdam, Netherlands),
H F Merk, and
H Mukhtar, and
B Schutte, and
I Kaufmann, and
M Das, and
D R Bickers
January 1990,
Archives of toxicology,
H F Merk, and
H Mukhtar, and
B Schutte, and
I Kaufmann, and
M Das, and
D R Bickers
January 2000,
Marine environmental research,
H F Merk, and
H Mukhtar, and
B Schutte, and
I Kaufmann, and
M Das, and
D R Bickers
July 1987,
Xenobiotica; the fate of foreign compounds in biological systems,
