Biomaterial Database

Effect of washing on sperm surface autoantibodies. 1987

A J Adeghe

Department of Obstetrics and Gynaecology, Birmingham Maternity Hospital.

This study tested the hypothesis that immediate washing "removes" sperm surface antibodies. Nine ejaculates with antisperm antibodies were studied. Separate portions of each ejaculate were washed by centrifugation (Shulman's method) as soon as possible after production (0-h) and 2 h later (2-h). In four samples, portions were also washed by a simple swim-up migration started at 0-h. Washed sperm were tested for surface antibodies using the immunobead binding test. The number of sperm with bound IgG antibodies at 0-h was significantly less than that at 2-h (P less than 0.001) but no significant difference was found in respect of IgA antibodies. Swim-up migration yielded results similar to those of centrifugation and had the advantage of selecting highly motile sperm. The potential application and implication of these findings are discussed.

UI	MeSH Term	Description	Entries
D007248	Infertility, Male	The inability of the male to effect FERTILIZATION of an OVUM after a specified period of unprotected intercourse. Male sterility is permanent infertility.	Sterility, Male,Sub-Fertility, Male,Subfertility, Male,Male Infertility,Male Sterility,Male Sub-Fertility,Male Subfertility,Sub Fertility, Male
D007315	Insemination, Artificial	Artificial introduction of SEMEN or SPERMATOZOA into the VAGINA to facilitate FERTILIZATION.	Artificial Insemination,Eutelegenesis,Artificial Inseminations,Eutelegeneses,Inseminations, Artificial
D007317	Insemination, Artificial, Homologous	Human artificial insemination in which the husband's semen is used.	Insemination, Artificial, Husband,AIH,Artificial Insemination, Husband,Homologous Insemination,Insemination, Homologous,Husband Artificial Insemination,Insemination, Husband Artificial
D008297	Male		Males
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001323	Autoantibodies	Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.	Autoantibody
D013094	Spermatozoa	Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.	Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms