Biomaterial Database

In vivo labeling of serotonin-containing neurons by 5,7-dihydroxytryptamine in Aplysia. 1987

B Jahan-Parwar, and K S Rozsa, and J Salanki, and M L Evans, and D O Carpenter

New York State Department of Health, Wadsworth Center for Laboratories and Research, University at Albany, NY 12201.

Intrahemocoelial administration of 5,7-dihydroxytryptamine (5,7-DHT) to Aplysia californica induces a transient (less than 4 h) behavioral alteration. About 5 weeks after 5,7-DHT treatment, 5-hydroxytryptamine (5-HT)-containing neurons develop dark brown pigmentation. These labeled 5-HT neurons have normal physiological and pharmacological properties when investigated electrophysiologically. This contrasts with the long-term neurotoxic effect of 5,7-DHT on vertebrate neurons. This technique will greatly facilitate visual identification of 5-HT-containing neurons and study of their physiology and actions.

UI	MeSH Term	Description	Entries
D009474	Neurons	The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.	Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D004102	Dihydroxytryptamines	Tryptamine substituted with two hydroxyl groups in any position. Some are cytotoxic serotonin analogs that are preferentially taken up by serotonergic neurons and then destroy those neurons.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001048	Aplysia	An opisthobranch mollusk of the order Anaspidea. It is used frequently in studies of nervous system development because of its large identifiable neurons. Aplysiatoxin and its derivatives are not biosynthesized by Aplysia, but acquired by ingestion of Lyngbya (seaweed) species.	Aplysias
D001522	Behavior, Animal	The observable response an animal makes to any situation.	Autotomy Animal,Animal Behavior,Animal Behaviors
D012701	Serotonin	A biochemical messenger and regulator, synthesized from the essential amino acid L-TRYPTOPHAN. In humans it is found primarily in the central nervous system, gastrointestinal tract, and blood platelets. Serotonin mediates several important physiological functions including neurotransmission, gastrointestinal motility, hemostasis, and cardiovascular integrity. Multiple receptor families (RECEPTORS, SEROTONIN) explain the broad physiological actions and distribution of this biochemical mediator.	5-HT,5-Hydroxytryptamine,3-(2-Aminoethyl)-1H-indol-5-ol,Enteramine,Hippophaine,Hydroxytryptamine,5 Hydroxytryptamine
D015116	5,7-Dihydroxytryptamine	Tryptamine substituted with two hydroxyl groups in positions 5 and 7. It is a neurotoxic serotonin analog that destroys serotonergic neurons preferentially and is used in neuropharmacology as a tool.	3-(2-Aminoethyl)-1H-indole-5,7-diol,5,7-Dihydroxytryptamine Creatine Sulfate,5,7 Dihydroxytryptamine,5,7 Dihydroxytryptamine Creatine Sulfate,Creatine Sulfate, 5,7-Dihydroxytryptamine,Sulfate, 5,7-Dihydroxytryptamine Creatine