BIOMAT Database Logo BIOMAT Database Logo

Isolation and Identification of Dendritic Cell Subsets from Human and Mouse Tumors. 2023

Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard, Lyon, France.

Dendritic cells (DCs) are professional antigen-presenting cells (APCs) that have the ability to orchestrate adaptive and innate immune responses by antigen phagocytosis and T cell activation across different inflammatory settings such as tumor development. As specific DC identity and how these cells interact with their neighbors is still not fully understood, it remains a challenge to unravel DC heterogeneity, particularly in human cancers. In this chapter, we describe a protocol to isolate and characterize tumor-infiltrating DCs.

UI MeSH Term Description Entries
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D009369 Neoplasms New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms. Benign Neoplasm,Cancer,Malignant Neoplasm,Tumor,Tumors,Benign Neoplasms,Malignancy,Malignant Neoplasms,Neoplasia,Neoplasm,Neoplasms, Benign,Cancers,Malignancies,Neoplasias,Neoplasm, Benign,Neoplasm, Malignant,Neoplasms, Malignant
D010587 Phagocytosis The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES). Phagocytoses
D003713 Dendritic Cells Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION). Dendritic Cells, Interdigitating,Interdigitating Cells,Plasmacytoid Dendritic Cells,Veiled Cells,Dendritic Cells, Interstitial,Dendritic Cells, Plasmacytoid,Interdigitating Dendritic Cells,Interstitial Dendritic Cells,Cell, Dendritic,Cell, Interdigitating,Cell, Interdigitating Dendritic,Cell, Interstitial Dendritic,Cell, Plasmacytoid Dendritic,Cell, Veiled,Cells, Dendritic,Cells, Interdigitating,Cells, Interdigitating Dendritic,Cells, Interstitial Dendritic,Cells, Plasmacytoid Dendritic,Cells, Veiled,Dendritic Cell,Dendritic Cell, Interdigitating,Dendritic Cell, Interstitial,Dendritic Cell, Plasmacytoid,Interdigitating Cell,Interdigitating Dendritic Cell,Interstitial Dendritic Cell,Plasmacytoid Dendritic Cell,Veiled Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
Isolation of Mouse Dendritic Cell Subsets and Macrophages from the Skin.
January 2016, Methods in molecular biology (Clifton, N.J.),
Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
Isolation of Human Skin Dendritic Cell Subsets.
January 2016, Methods in molecular biology (Clifton, N.J.),
Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
Review of Mouse and Human Dendritic Cell Subsets.
January 2016, Methods in molecular biology (Clifton, N.J.),
Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men.
January 2016, Methods in molecular biology (Clifton, N.J.),
Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
From skin dendritic cells to a simplified classification of human and mouse dendritic cell subsets.
August 2010, European journal of immunology,
Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
Study and isolation of human intestinal dendritic cell and macrophage subsets.
January 2023, Methods in cell biology,
Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
Differentiation of dendritic cell subsets from mouse bone marrow.
January 2007, Methods in molecular biology (Clifton, N.J.),
Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets.
April 2016, Journal of visualized experiments : JoVE,
Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
Human dendritic cell subsets.
September 2013, Immunology,
Yamila Rocca, and Aurélien Voissière, and Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare
Isolation of Conventional Murine Lung Dendritic Cell Subsets.
January 2022, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!