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BACKGROUND D4-androstenedione (D4ASD) is an intermediate hormone of androgen biosynthesis by the gonads and the adrenal glands. The interest in D4ASD concentration assessment resides in diagnostics of androgenic hyperproduction pathologies. Currently, many D4ASD quantification methods are available on the market including immunological methods that remain problematic due to the possible cross-reactivity with endogenous or exogenous steroids. METHODS Recently Roche® launched a new fully automated instrument for the measurement of D4ASD concentration. In this paper, the criteria of analytical performance (repeatability and intermediate precision) of the D4ASD Roche® assay were assessed and compared with 2 different methods including a radioimmunoassay (RIA) as well as a liquid chromatography tandem mass spectrometry (LC-MS/MS) method. RESULTS Repeatability and intermediate precision of the D4ASD Roche® were acceptable according to the prede-fined RICOS standard (CV ≤ 7.9%) and the assay showed a good correlation with other assays considering the 95% CI obtained for the slope and the y-intercept. CONCLUSIONS This method demonstrates acceptable criteria of analytical performance with an intermediate imprecision and a trueness within the fixed acceptance limits.
Isabelle Ruth, and
Caroline Le Goff, and
Stephanie Peeters, and
Leonidas Bizumukama, and
Guillaume Deprez, and
Cecile Duterme, and
Frederic Cotton, and
Fleur Wolff
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Isabelle Ruth, and
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Stephanie Peeters, and
Leonidas Bizumukama, and
Guillaume Deprez, and
Cecile Duterme, and
Frederic Cotton, and
Fleur Wolff
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Isabelle Ruth, and
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Practical laboratory medicine,
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Guillaume Deprez, and
Cecile Duterme, and
Frederic Cotton, and
Fleur Wolff
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Clinical chemistry,
Isabelle Ruth, and
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International journal of laboratory hematology,
