Escherichia coli O157:H7 expresses extracellular proteins called curli that are essential for surface colonization. Transfer rates of E. coli O157:H7 0018+ (curli+), and 0018- (curli-) from inoculated to noninoculated lettuce pieces during washing were quantified in this study. Romaine lettuce pieces were inoculated with ∼6 log CFU on just the surface, just the cut edges, or both surface and cut edges. Samples were dried for 2 h in a biosafety cabinet and then washed with ten (10) noninoculated lettuce pieces in 500 mL of water for 30 s. The curli- strain was more readily removed (3 log reduction) compared to the curli+ (1 log reduction) when only the lettuce surface was inoculated (p > 0.05). The same was true when only the lettuce piece edge was inoculated (p > 0.05), although the magnitude of the reduction was less. There was no significant difference in reduction of curli+ strain between any of the surfaces. There was a significant difference (p < 0.05) in reduction of the curli- strains when comparing the leaf surface (more removal) to the cut leaf edge (less removal). The curli+ strain always showed significantly (p < 0.05) more transfer to noninoculated leaves regardless of the inoculation location. The curli+ strain transferred about -1 log percent (∼0.1%) to noninoculated pieces, while the curli- strain transferred about -2 log percent (∼0.01%) CFU to the noninoculated pieces. Mean log percent transfer was not significantly different within the curli+ or curli- experiments (p > 0.05). When the leaf surface was inoculated, there was about 2 log percent (i.e., close to 100% transfer) into the wash water for both the curli+ and curli- strains. When only the cut edges or surface and edge were inoculated, observed mean transfer rates were lower but not significantly different (p > 0.05). Further research is needed to more fully understand the factors that influence bacterial cross-contamination during the washing of fresh produce.