Mitochondrial-derived vesicles retain membrane potential and contain a functional ATP synthase. 2023

Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
Department of Molecular Genetics and Microbiology, IMRIC, Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Vesicular transport is a means of communication. While cells can communicate with each other via secretion of extracellular vesicles, less is known regarding organelle-to organelle communication, particularly in the case of mitochondria. Mitochondria are responsible for the production of energy and for essential metabolic pathways in the cell, as well as fundamental processes such as apoptosis and aging. Here, we show that functional mitochondria isolated from Saccharomyces cerevisiae release vesicles, independent of the fission machinery. We isolate these mitochondrial-derived vesicles (MDVs) and find that they are relatively uniform in size, of about 100 nm, and carry selective protein cargo enriched for ATP synthase subunits. Remarkably, we further find that these MDVs harbor a functional ATP synthase complex. We demonstrate that these vesicles have a membrane potential, produce ATP, and seem to fuse with naive mitochondria. Our findings reveal a possible delivery mechanism of ATP-producing vesicles, which can potentially regenerate ATP-deficient mitochondria and may participate in organelle-to-organelle communication.

UI MeSH Term Description Entries
D008564 Membrane Potentials The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization). Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D008928 Mitochondria Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed) Mitochondrial Contraction,Mitochondrion,Contraction, Mitochondrial,Contractions, Mitochondrial,Mitochondrial Contractions
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D001692 Biological Transport The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments. Transport, Biological,Biologic Transport,Transport, Biologic
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker

Related Publications

Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
January 2004, Toxicology mechanisms and methods,
Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
February 2013, Histochemistry and cell biology,
Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
April 1999, Biochemical and biophysical research communications,
Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
May 2006, The Journal of biological chemistry,
Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
January 2006, Eukaryotic cell,
Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
August 1991, Proceedings of the National Academy of Sciences of the United States of America,
Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
July 2022, International journal of molecular sciences,
Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
January 1997, European journal of biochemistry,
Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
July 2020, Mitochondrion,
Reut Hazan Ben-Menachem, and Dvora Lintzer, and Tamar Ziv, and Koyeli Das, and Irit Rosenhek-Goldian, and Ziv Porat, and Hila Ben Ami Pilo, and Sharon Karniely, and Ann Saada, and Neta Regev-Rudzki, and Ophry Pines
April 2008, The EMBO journal,
Copied contents to your clipboard!