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Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection. 2023
Amanda Labora, and
Hailey Lee, and
Charlotte Chan, and
Erin Tabornal, and
Thuc Le, and
Khalid Rashid, and
Evan Abt, and
Takanobu Yamao, and
Hanna Mandl, and
Amanda Creech, and
Alykhan Premji, and
Luyi Li, and
Jason Link, and
Nanping Wu, and
Caius Radu, and
Timothy Donahue
Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: alabora@mednet.ucla.edu.
Here, we present a protocol to generate a murine model of liver metastasis by directly injecting tumor cells into the portal vein under ultrasound guidance. We describe steps for animal and cell preparation and two techniques for injecting tumor cells. One technique is freehand, while the other technique is device-assisted using a 3D-printed prototype device. Finally, we describe tumor surveillance with bioluminescent imaging.
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