Biomaterial Database

Effects of cytochalasin B on cell to cell adhesion and cellular shape of embryo mesoderm cells in vitro. 1986

C A Chamorro, and P de Paz Cabello, and J G Fernandez, and J M Villar

Departamento de Anatomía Embriología, Facultad de Veterinaria, Universidad de León, Spain.

The effects of cytochalasin B on chick embryo mesoderm cells cultured in vitro were studied by scanning electron microscopy. The untreated cells showed numerous filopodia and lamellipodia and they were flattened onto the coverslip. Several cellular clusters were observed in each culture. The treated cells did not show filopodia, they had a rounded shape and cellular clusters were not present on the coverslip. These alterations are discussed on the basis of the actions of cytochalasin B on the micrcfilamentous cytoskeleton and the role proposed for microfilaments in filopodia formation, cellular shape and locomotion. It was observed that the mechanisms of cell to cell adhesion of chick embryo mesoderm cells were not the same as the mechanisms of cell adhesion to artificial substrates.

UI	MeSH Term	Description	Entries
D008648	Mesoderm	The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube.	Mesenchyme,Dorsal Mesoderm,Intermediate Mesoderm,Lateral Plate Mesoderm,Mesenchyma,Paraxial Mesoderm,Dorsal Mesoderms,Intermediate Mesoderms,Lateral Plate Mesoderms,Mesenchymas,Mesoderm, Dorsal,Mesoderm, Intermediate,Mesoderm, Lateral Plate,Mesoderm, Paraxial,Mesoderms, Dorsal,Mesoderms, Intermediate,Mesoderms, Lateral Plate,Mesoderms, Paraxial,Paraxial Mesoderms,Plate Mesoderm, Lateral,Plate Mesoderms, Lateral
D002448	Cell Adhesion	Adherence of cells to surfaces or to other cells.	Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002642	Chick Embryo	The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.	Embryo, Chick,Chick Embryos,Embryos, Chick
D003571	Cytochalasin B	A cytotoxic member of the CYTOCHALASINS.	Phomin
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In