Biomaterial Database

Spinocerebellar projections from the central cervical nucleus in the cat, as studied by anterograde transport of wheat germ agglutinin-horseradish peroxidase. 1987

M Matsushita, and T Tanami

Department of Anatomy, University of Tsukuba, Ibaraki, Japan.

The projection of the spinocerebellar tract arising from the central cervical nucleus with crossed ascending axons was studied by the anterograde transport of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) in the cat. Following injections of WGA-HRP into the C1 to the C4 or C5 segments, labeled terminals were seen in lobules I-VI, sublobule VIIb, lobules VIII and IX, the paramedian lobule, crus I and crus II, and the simple lobule. About 67-80% of the total number of labeled terminals were in the anterior lobe and 20-33% in the posterior lobe; the labeled terminals were abundant in lobules I (11-20%), II (11-28%), III (5.7-9.6%), IV (8.6-15.4%), and VIII (9-12%). The labeled terminals were densely distributed in the basal half or basal two-thirds of sublobules IIa-Va and VIf-VIb in their apicobasal extent and the transitional areas between neighboring sublobules. In sublobules Ia, Ib, and Vg they were distributed over the entire sublobule. The labeled terminals were most abundant within 0.5 mm of the midline (30-55% of the total number in each sublobule). Results in cases with injections into the C2-C4 segments, preceded by hemisection between the C1 and C2 segments, revealed that the projections were bilateral but predominantly contralateral to the cells of origin; the proportion of the quantity of the contralateral projection to that of the ipsilateral was about 60:40% in sublobules Ia-Vb and sublobules Ve-Vg. The projection field in the horizontal plane of the lobule was reconstructed from a series of cross sections through each sublobule. The labeled terminals were distributed in three major longitudinal areas named areas 1, 2, and 3, respectively. These areas were confined in the basal half to two-thirds of lobules III-V: area 1 located in zone A1 of Voogd (within 0.25 mm of the midline); area 2 located in zones A1 to A2 (at around 0.5 mm lateral to the midline); and area 3 located in the lateral part of zone A2 to zone B (between 0.75 mm and 1.5 mm lateral to the midline in lobule III, and between 1.0 mm and 2.0 mm lateral to the midline in lobules IV-VI). An indefinite area 4 appeared in zones B and C of some lobules. In sublobules Ia, Ib, Vf, and Vg the three areas extended throughout the apicobasal length.(ABSTRACT TRUNCATED AT 400 WORDS)

UI	MeSH Term	Description	Entries
D009434	Neural Pathways	Neural tracts connecting one part of the nervous system with another.	Neural Interconnections,Interconnection, Neural,Interconnections, Neural,Neural Interconnection,Neural Pathway,Pathway, Neural,Pathways, Neural
D002415	Cats	The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)	Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D002525	Cerebellar Cortex	The superficial GRAY MATTER of the CEREBELLUM. It consists of two main layers, the stratum moleculare and the stratum granulosum.	Cortex Cerebelli,Cerebelli, Cortex,Cerebellus, Cortex,Cortex Cerebellus,Cortex, Cerebellar
D006735	Horseradish Peroxidase	An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.	Alpha-Peroxidase,Ferrihorseradish Peroxidase,Horseradish Peroxidase II,Horseradish Peroxidase III,Alpha Peroxidase,II, Horseradish Peroxidase,III, Horseradish Peroxidase,Peroxidase II, Horseradish,Peroxidase III, Horseradish,Peroxidase, Ferrihorseradish,Peroxidase, Horseradish
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013116	Spinal Cord	A cylindrical column of tissue that lies within the vertebral canal. It is composed of WHITE MATTER and GRAY MATTER.	Coccygeal Cord,Conus Medullaris,Conus Terminalis,Lumbar Cord,Medulla Spinalis,Myelon,Sacral Cord,Thoracic Cord,Coccygeal Cords,Conus Medullari,Conus Terminali,Cord, Coccygeal,Cord, Lumbar,Cord, Sacral,Cord, Spinal,Cord, Thoracic,Cords, Coccygeal,Cords, Lumbar,Cords, Sacral,Cords, Spinal,Cords, Thoracic,Lumbar Cords,Medulla Spinali,Medullari, Conus,Medullaris, Conus,Myelons,Sacral Cords,Spinal Cords,Spinali, Medulla,Spinalis, Medulla,Terminali, Conus,Terminalis, Conus,Thoracic Cords
D014909	Wheat Germ Agglutinins	Lectins purified from the germinating seeds of common wheat (Triticum vulgare); these bind to certain carbohydrate moieties on cell surface glycoproteins and are used to identify certain cell populations and inhibit or promote some immunological or physiological activities. There are at least two isoforms of this lectin.	Agglutinins, Wheat Germ,Lectins, Triticum Vulgare,Lectins, Wheat Germ,Triticum Vulgare Lectin,Triticum Vulgare Lectins,Wheat Germ Agglutinin,Wheat Germ Lectin,Wheat Germ Lectins,Wheat Germ Agglutinin Isolectin 1,Wheat Germ Agglutinin Isolectin 2,Agglutinin, Wheat Germ,Germ Agglutinin, Wheat,Germ Lectin, Wheat,Lectin, Triticum Vulgare,Lectin, Wheat Germ,Vulgare Lectin, Triticum