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A radioassay for the measurement of cortisol binding capacity in human serum was developed and the results validated by radioimmunoassay of serum corticosteroid binding globulin. The mini-scale method is simple, reliable and rapid, and avoids separation of partitioned phases. The linear correlation between the free cortisol index and free cortisol concentration as calculated by the two methods utilizing serum samples with normal and abnormal protein concentrations was r = 0.962 (n = 114, p less than 0.001) and the regression equation was y = 3.516 x - 2.632. The technique requires no preparation of reagents, and the assay can be performed in 45 minutes using 0.05 ml of serum or plasma.
R S Salem, and
M Hassan, and
M A Al-Awqati, and
M Abu-Omeir, and
D Issac, and
H M Abdul-Dayem
February 1980,
Clinical chemistry,
R S Salem, and
M Hassan, and
M A Al-Awqati, and
M Abu-Omeir, and
D Issac, and
H M Abdul-Dayem
January 1969,
Annales d'endocrinologie,
R S Salem, and
M Hassan, and
M A Al-Awqati, and
M Abu-Omeir, and
D Issac, and
H M Abdul-Dayem
November 1974,
Clinical chemistry,
R S Salem, and
M Hassan, and
M A Al-Awqati, and
M Abu-Omeir, and
D Issac, and
H M Abdul-Dayem
June 1969,
Clinical chemistry,
R S Salem, and
M Hassan, and
M A Al-Awqati, and
M Abu-Omeir, and
D Issac, and
H M Abdul-Dayem
January 1975,
Journal of medicine,
R S Salem, and
M Hassan, and
M A Al-Awqati, and
M Abu-Omeir, and
D Issac, and
H M Abdul-Dayem
March 1968,
The Journal of clinical endocrinology and metabolism,
R S Salem, and
M Hassan, and
M A Al-Awqati, and
M Abu-Omeir, and
D Issac, and
H M Abdul-Dayem
January 1973,
Nouvelle revue francaise d'hematologie,
R S Salem, and
M Hassan, and
M A Al-Awqati, and
M Abu-Omeir, and
D Issac, and
H M Abdul-Dayem
November 1996,
Ginekologia polska,
R S Salem, and
M Hassan, and
M A Al-Awqati, and
M Abu-Omeir, and
D Issac, and
H M Abdul-Dayem
January 2012,
Iranian journal of pharmaceutical research : IJPR,
R S Salem, and
M Hassan, and
M A Al-Awqati, and
M Abu-Omeir, and
D Issac, and
H M Abdul-Dayem
October 1975,
Endocrinology,
