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Glucocorticosteroids stimulate growth hormone (GH) synthesis and inhibit prolactin (PRL) synthesis and cell growth in cultured GH3 cells, a clonal cell strain derived from a rat pituitary tumour. This model system was used to study the mechanism by which glucocorticosteroids enter target cells. The cellular uptake of [3H]dexamethasone was temperature dependent and was further inhibited by addition of an excess amount of cold dexamethasone. Half maximal uptake was obtained after about 5 min at 37 degrees C. The initial rates of [3H]dexamethasone uptake were a linear function of the extracellular hormone concentration. The uptake of [3H]dexamethasone in intact cells studied at different temperatures resulted in linear Arrhenius plots, with a calculated energy of activation of 91.0 kJ x mole-1 x degree-1. Scatchard analysis of specifically cell bound [3H]dexamethasone at equilibrium (0 degrees C) showed a straight line with a calculated dissociation constant (Kd) of 1.6 x 10(-9) M and a maximal uptake of 180 x 10(-15) mole/mg cell protein. Specific binding of [3H]dexamethasone to cytosol proteins could only be demonstrated at 0 degrees C. These results indicate that [3H]dexamethasone diffuses passively into the cell, and binds to specific receptors in an energy dependent way.
K Furu, and
K Kilvik, and
K M Gautvik, and
E Haug
August 1977,
Cell and tissue research,
K Furu, and
K Kilvik, and
K M Gautvik, and
E Haug
June 1974,
Lloydia,
K Furu, and
K Kilvik, and
K M Gautvik, and
E Haug
September 1973,
The Journal of biological chemistry,
K Furu, and
K Kilvik, and
K M Gautvik, and
E Haug
February 1982,
Molecular and cellular biology,
K Furu, and
K Kilvik, and
K M Gautvik, and
E Haug
September 1988,
Journal of molecular endocrinology,
K Furu, and
K Kilvik, and
K M Gautvik, and
E Haug
January 1982,
Journal of steroid biochemistry,
K Furu, and
K Kilvik, and
K M Gautvik, and
E Haug
March 1982,
Endocrinology,
K Furu, and
K Kilvik, and
K M Gautvik, and
E Haug
February 1990,
Planta medica,
K Furu, and
K Kilvik, and
K M Gautvik, and
E Haug
December 1994,
Endocrinology,
K Furu, and
K Kilvik, and
K M Gautvik, and
E Haug
May 1997,
Endocrinology,
