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Increased lentivirus titer using ultra expression vectors. 2023
So Fujibayashi, and
Tohru Kiyono, and
Yuka Endo, and
Tetsuya Tani, and
Haruka Tate, and
Lanlan Bai, and
Eriko Sugano, and
Hiroshi Tomita, and
Tomokazu Fukuda
Graduate School of Science and Engineering, Iwate University, 4-3-5, Ueda, Morioka, Iwate, 020-8551, Japan.
Lentivirus is an efficient gene transfer system that is widely used in basic science. We aimed to improve viral titer by applying an ultra-expression vectors to lentiviral packaging. Application of the ultra-expression vectors increased biological titer 4 times for standard preparation. We also evaluated the efficacy of the ultra-expression vectors to relatively longer insert fragments, such as CSII-CMV-CNROE containing 5 genes in multiple cloning sites. Packaging of the ultra-expression vectors showed 3.5 times higher biological titer compared with the original method. Our improved packaging system could be applied to lentivirus to produce higher titers.
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