BIOMAT Database Logo BIOMAT Database Logo

Specificity and functions of guanine methylase of Shigella sonnei DDVI phage. 1979

I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov

DNA methylase methylating adenine with formation of 6-methylaminopurine has been identified in Shigella sonnei 1188 cells which are the natural host of DDVI phage. At the same time, in DNA of DDVI phage replicating both in Sh. sonnei 1188 cells and in Escherichia coli B cells 7-methylguanine was found as the only minor base in amounts of 0.25 and 0.27 mol per 100 mol of nucleotides, respectively. The extract of the infected cells was found to contain both kinds of DNA methylases: virus-specific guanine methylase and cellular adenine methylase. The lack of 6-methylaminopurine in DNA of this phage is explained by reversible inhibition of the cell enzyme in the infected cells. The amount of methyl groups transferred by DDVI-specific methylase on DNA does not depend on the species of the infected cells and is similar in the case of unmodified SD phage DNA and DNA of T2 phage methylated by E. coli B enzyme. Guanine methylase has been shown to be a DDVI-induced modification enzyme and to protect against restriction of B-type. It methylates double-stranded DNAs only and is inhibited by S-adenosylhomocysteine.

UI MeSH Term Description Entries
D008780 Methyltransferases A subclass of enzymes of the transferase class that catalyze the transfer of a methyl group from one compound to another. (Dorland, 28th ed) EC 2.1.1. Methyltransferase
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D003848 Deoxyguanine Nucleotides Guanine nucleotides which contain deoxyribose as the sugar moiety. Deoxyguanosine Phosphates,Nucleotides, Deoxyguanine,Phosphates, Deoxyguanosine
D004248 DNA (Cytosine-5-)-Methyltransferases Enzymes that catalyzes the transfer of a methyl group from S-ADENOSYLMETHIONINE to the 5-position of CYTOSINE residues in DNA. DNA (Cytosine-5-)-Methyltransferase,DNA Cytosine-5-Methylase,DNA (Cytosine 5) Methyltransferase,Cytosine-5-Methylase, DNA,DNA Cytosine 5 Methylase
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006147 Guanine
D001435 Bacteriophages Viruses whose hosts are bacterial cells. Phages,Bacteriophage,Phage
D012764 Shigella sonnei A lactose-fermenting bacterium causing dysentery. Bacterium sonnei
D013329 Structure-Activity Relationship The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups. Relationship, Structure-Activity,Relationships, Structure-Activity,Structure Activity Relationship,Structure-Activity Relationships
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities

Related Publications

I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov
Methylated guanine derivative as a minor base in the DNA of phage DDVI Shigella disenteriae.
June 1976, Biochimica et biophysica acta,
I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov
Phage typing of Shigella sonnei.
January 1968, Archivum immunologiae et therapiae experimentalis,
I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov
Phage typing of Shigella sonnei.
January 1968, Archivum immunologiae et therapiae experimentalis,
I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov
Phage typing of Shigella sonnei.
January 1973, Archivum immunologiae et therapiae experimentalis,
I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov
[Phage typing of Shigella sonnei].
February 1958, Das Deutsche Gesundheitswesen,
I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov
Shigella sonnei phage typing Czechoslovakia.
January 1973, Journal of hygiene, epidemiology, microbiology, and immunology,
I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov
To Shigella sonnei phage typing.
January 1977, Journal of hygiene, epidemiology, microbiology, and immunology,
I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov
[Modifying methylase SsoI from Shigella sonnei 47 cells].
October 1988, Molekuliarnaia genetika, mikrobiologiia i virusologiia,
I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov
Phage and colicin typing of Shigella sonnei.
January 1969, Acta microbiologica Academiae Scientiarum Hungaricae,
I I Nikolskaya, and M I Tediashvili, and N G Lopatina, and T G Chanishvili, and S S Debov
Attempts at phage typing of Shigella sonnei.
January 1967, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. Originale,
Copied contents to your clipboard!