BIOMAT Database Logo BIOMAT Database Logo

Full-length EFOP3 and EFOP4 proteins are essential for pollen intine development in Arabidopsis thaliana. 2023

Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Rice Research Institute, Chongqing, 400715, People's Republic of China.

Pollen development is critical to plant reproduction, but the underlying regulatory molecular mechanisms have not been fully elucidated. The Arabidopsis (Arabidopsis thaliana) EFR3 OF PLANT 3 (EFOP3) and EFR3 OF PLANT 4 (EFOP4) genes encode members of the Armadillo (ARM) repeat superfamily that play key roles in pollen development. Herein, we demonstrate that EFOP3 and EFOP4 are co-expressed in pollen at anther stages 10-12, but loss-of-function of both EFOP3 and EFOP4 leads to male gametophyte sterility, irregular intine, and shriveled pollen grains at anther stage 12. We further established that full-length EFOP3 and EFOP4 specifically localize to the plasma membrane, and the integrity of these proteins is essential for pollen development. We observed uneven intine, less organized cellulose and reduced pectin content in mutant pollen compared with the wild-type. These, together with the misexpression of several genes related to cell wall metabolism in efop3-/- efop4+/- mutants, suggest that EFOP3 and EFOP4 may indirectly regulate the expression of these genes to affect intine formation, thus controlling Arabidopsis pollen fertility in a functionally redundant manner. Moreover, transcriptome analysis showed that the absence of EFOP3 and EFOP4 function affects multiple pollen development pathways. These results enhance our understanding of EFOPs proteins and their role in pollen development.

UI MeSH Term Description Entries
D011058 Pollen The fertilizing element of plants that contains the male GAMETOPHYTES. Male Gametes, Plant,Male Gametophytes,Microspores, Plant,Plant Microspores,Pollen Grains,Gamete, Plant Male,Gametes, Plant Male,Gametophyte, Male,Gametophytes, Male,Grain, Pollen,Grains, Pollen,Male Gamete, Plant,Male Gametophyte,Microspore, Plant,Plant Male Gamete,Plant Male Gametes,Plant Microspore,Pollen Grain
D012098 Reproduction The total process by which organisms produce offspring. (Stedman, 25th ed) Human Reproductive Index,Human Reproductive Indexes,Reproductive Period,Human Reproductive Indices,Index, Human Reproductive,Indexes, Human Reproductive,Indices, Human Reproductive,Period, Reproductive,Periods, Reproductive,Reproductive Index, Human,Reproductive Indices, Human,Reproductive Periods
D005298 Fertility The capacity to conceive or to induce conception. It may refer to either the male or female. Fecundity,Below Replacement Fertility,Differential Fertility,Fecundability,Fertility Determinants,Fertility Incentives,Fertility Preferences,Fertility, Below Replacement,Marital Fertility,Natural Fertility,Subfecundity,World Fertility Survey,Determinant, Fertility,Determinants, Fertility,Fertility Determinant,Fertility Incentive,Fertility Preference,Fertility Survey, World,Fertility Surveys, World,Fertility, Differential,Fertility, Marital,Fertility, Natural,Preference, Fertility,Preferences, Fertility,Survey, World Fertility,Surveys, World Fertility,World Fertility Surveys
D017360 Arabidopsis A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development. Arabidopsis thaliana,Cress, Mouse-ear,A. thaliana,A. thalianas,Arabidopses,Arabidopsis thalianas,Cress, Mouse ear,Cresses, Mouse-ear,Mouse-ear Cress,Mouse-ear Cresses,thaliana, A.,thaliana, Arabidopsis,thalianas, A.
D018506 Gene Expression Regulation, Plant Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants. Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant
D029681 Arabidopsis Proteins Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments. Arabidopsis thaliana Proteins,Thale Cress Proteins,Proteins, Arabidopsis thaliana,thaliana Proteins, Arabidopsis

Related Publications

Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
Arabidopsis FAB1/PIKfyve proteins are essential for development of viable pollen.
December 2009, Plant physiology,
Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
Development and genetic regulation of pollen intine in Arabidopsis and rice.
January 2024, Gene,
Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
An Arabidopsis thaliana plasma membrane proton pump is essential for pollen development.
November 2004, Genetics,
Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
RHO OF PLANT proteins are essential for pollen germination in Arabidopsis.
August 2023, Plant physiology,
Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
Pollen-Specific Aquaporins NIP4;1 and NIP4;2 Are Required for Pollen Development and Pollination in Arabidopsis thaliana.
May 2016, The Plant cell,
Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
Cajal bodies are developmentally regulated during pollen development and pollen tube growth in Arabidopsis thaliana.
July 2013, Molecular plant,
Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
Arabidopsis reversibly glycosylated polypeptides 1 and 2 are essential for pollen development.
December 2006, Plant physiology,
Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
Annexin5 is essential for pollen development in Arabidopsis.
April 2014, Molecular plant,
Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
AtMYB32 is required for normal pollen development in Arabidopsis thaliana.
December 2004, The Plant journal : for cell and molecular biology,
Xinlong Chen, and Yingying Zhang, and Wuzhong Yin, and Gang Wei, and Hailing Xu, and Lu Ma, and Weijiang Tian, and Guang Yang, and Yunfeng Li, and Renhong Wu, and Ting Zhang, and Nan Wang, and Guanghua He
Proteins are polyisoprenylated in Arabidopsis thaliana.
September 2004, Biochemical and biophysical research communications,
Copied contents to your clipboard!