Facile transduction with P1 phage in Escherichia coli associated with urinary tract infections. 2023

Jacob Hogins, and Sushmita Sudarshan, and Philippe Zimmern, and Larry Reitzer
Department of Biological Sciences, University of Texas at Dallas, 800 West Campbell Road, Richardson, TX 75080-3021, United States of America. Electronic address: Jxh150330@utdallas.edu.

We describe a modification of a standard method that efficiently transduces genes using P1 phage into uropathogenic Escherichia coli strains. This procedure allows utilization of the large KEIO mutant library for analysis of pathogenic E. coli strains.

UI MeSH Term Description Entries
D004927 Escherichia coli Infections Infections with bacteria of the species ESCHERICHIA COLI. E coli Infections,E. coli Infection,Infections, E coli,Infections, Escherichia coli,E coli Infection,E. coli Infections,Escherichia coli Infection,Infection, E coli,Infection, E. coli,Infection, Escherichia coli
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014552 Urinary Tract Infections Inflammatory responses of the epithelium of the URINARY TRACT to microbial invasions. They are often bacterial infections with associated BACTERIURIA and PYURIA. Infection, Urinary Tract,Infections, Urinary Tract,Tract Infection, Urinary,Tract Infections, Urinary,Urinary Tract Infection
D017101 Bacteriophage P1 A species of temperate bacteriophage in the genus P1-like viruses, family MYOVIRIDAE, which infects E. coli. It is the largest of the COLIPHAGES and consists of double-stranded DNA, terminally redundant, and circularly permuted. Coliphage P1,Enterobacteria phage P1,P1 Phage,Phage P1,P1 Phages,Phage, P1,Phages, P1
D056689 Uropathogenic Escherichia coli Strains of Escherichia coli that preferentially grow and persist within the urinary tract. They exhibit certain virulence factors and infection pathways that cause URINARY TRACT INFECTIONS. Uropathogenic E. coli
D029968 Escherichia coli Proteins Proteins obtained from ESCHERICHIA COLI. E coli Proteins

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