OBJECTIVE To compare the amount of abrasion of four whitening toothpastes, two conventional toothpastes, and seven experimental toothpastes with varying concentrations of hydrogen peroxide. METHODS Bovine dentin specimens were treated with the four whitening toothpastes (containing three different concentrations of hydrogen peroxide: 0.75%, 1.50%, and 2.80%), two conventional toothpastes without hydrogen peroxide, seven experimental toothpastes (concentrations of hydrogen peroxide: 0.75%, 1.50%, 3.0%, 4.50%, 6.0%, 7.50%, and 9.0%), and distilled water. After 10,000 strokes of toothbrushing, the amount of abrasion on the dentin surface was measured with a contactless 3D surface profiler (n= 8). The pH of all solutions, the weight percentages of the particles, and the component of particles in the toothpaste were analyzed. The correlations between the dentin abrasion, pH, and weight percentages of the particles in the toothpastes were investigated. RESULTS The amount of abrasion of the two conventional toothpastes were 1.1-3.6 times higher than those of the four whitening toothpastes. Likewise, the pH of the conventional toothpaste was higher than those of the other whitening toothpastes. No significant differences were found among the four whitening toothpastes. The four whitening toothpastes consisted of a relatively lower weight percentage of particles compared to the two conventional toothpastes. A strong positive correlation was observed between the dentin abrasion and the weight percentages of the particles (r= 0.913; P< 0.05). Furthermore, no significant differences in the amount of abrasion were observed between the specimens treated with seven experimental toothpastes and those treated with distilled water. CONCLUSIONS The whitening toothpastes containing less than 9% hydrogen peroxide did not seem to harm the dentin surface significantly. These findings can serve as a reference for consumers, patients, and dental professionals.