[Isolation of hexokinase II isoenzyme from the hyaloplasm of rat skeletal muscle]. 1986

E M Muntian, and N Iu Goncharova

A method for obtaining partially purified preparation of II isozyme of hexokinase from cytosol of the rat skeletal muscles has been suggested. According to the data of electrophoretic and kinetic analyses the preparation does not practically contain I isozyme of hexokinase and is characterized by high enzymatic activity. The obtained preparation of II isozyme of hexokinase may be successfully used for research of the adsorption mechanism controlling the enzyme activity.

UI MeSH Term Description Entries
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D007700 Kinetics The rate dynamics in chemical or physical systems.
D009132 Muscles Contractile tissue that produces movement in animals. Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D003593 Cytoplasm The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990) Protoplasm,Cytoplasms,Protoplasms
D006593 Hexokinase An enzyme that catalyzes the conversion of ATP and a D-hexose to ADP and a D-hexose 6-phosphate. D-Glucose, D-mannose, D-fructose, sorbitol, and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase. (From Enzyme Nomenclature, 1992) EC 2.7.1.1. Hexokinase A,Hexokinase D,Hexokinase II
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

E M Muntian, and N Iu Goncharova
January 1982, Methods in enzymology,
E M Muntian, and N Iu Goncharova
May 1991, Biokhimiia (Moscow, Russia),
E M Muntian, and N Iu Goncharova
March 1994, Biokhimiia (Moscow, Russia),
E M Muntian, and N Iu Goncharova
February 1967, The Journal of biological chemistry,
E M Muntian, and N Iu Goncharova
September 1997, Biochemical and biophysical research communications,
E M Muntian, and N Iu Goncharova
September 1988, FEBS letters,
E M Muntian, and N Iu Goncharova
August 1996, Journal of applied physiology (Bethesda, Md. : 1985),
E M Muntian, and N Iu Goncharova
February 1976, FEBS letters,
E M Muntian, and N Iu Goncharova
August 1987, Archives of biochemistry and biophysics,
E M Muntian, and N Iu Goncharova
January 1973, Biokhimiia (Moscow, Russia),
Copied contents to your clipboard!