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A procedure is described which separates neutral glycolipids from gangliosides, and which separates the gangliosides into classes, based on their number of sialic acid residues. In addition to separation into mono-, di- and trisialoganglioside classes, there is purification of individual disialoganglioside species. The procedure uses a commercially available--NH2 high performance liquid chromatography (HPLC) column, which normally is used as an adsorption column. In this method the column is modified by protonation, to pH 5.4, so that it exhibits ion exchange as well as adsorption properties. Sensitive, nondestructive detection of eluent peaks is accomplished by monitoring continuously at 210 nm, a wavelength near which underivatized glycosphingolipids have absorbance maxima and which also will clearly detect most possible contaminants. An entire run, including re-equilibration of the column, takes two hours.
M M Whalen, and
G C Wild, and
W D Spall, and
R J Sebring
November 1987,
Journal of chromatography,
M M Whalen, and
G C Wild, and
W D Spall, and
R J Sebring
August 1994,
Journal of chromatography. B, Biomedical applications,
M M Whalen, and
G C Wild, and
W D Spall, and
R J Sebring
January 1981,
Hemoglobin,
M M Whalen, and
G C Wild, and
W D Spall, and
R J Sebring
October 1982,
Journal of chromatography,
M M Whalen, and
G C Wild, and
W D Spall, and
R J Sebring
July 1979,
Journal of chromatography,
M M Whalen, and
G C Wild, and
W D Spall, and
R J Sebring
January 1995,
Nucleic acids symposium series,
M M Whalen, and
G C Wild, and
W D Spall, and
R J Sebring
April 1988,
The Analyst,
M M Whalen, and
G C Wild, and
W D Spall, and
R J Sebring
August 1983,
Journal of chromatography,
M M Whalen, and
G C Wild, and
W D Spall, and
R J Sebring
January 2014,
Methods in molecular biology (Clifton, N.J.),
M M Whalen, and
G C Wild, and
W D Spall, and
R J Sebring
October 1983,
Analytical biochemistry,
