BIOMAT Database Logo BIOMAT Database Logo

Community Synergy of Lactic Acid Bacteria and Cleaner Fermentation of Oat Silage Prepared with a Multispecies Microbial Inoculant. 2023

Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Inner Mongolia Engineering Research Center of Development and Utilization of Microbial Resources in Silage, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot, Inner Mongolia, People's Republic of China.

To investigate community synergy of lactic acid bacteria (LAB) and cleaner fermentation of oat silage, oat silages were prepared with or without (control) commercial LAB inoculants LI1 (containing Lactiplantibacillus plantarum, Lentilactobacillus buchneri, Lacticaseibacillus paracasei, and Pediococcus acidilactici) and LI2 (containing Lactiplantibacillus plantarum and Lentilactobacillus buchneri). The microbial community, LAB synergy, and cleaner fermentation were analyzed at 1, 3, 6, 15, 35, and 90 days of ensiling. The LAB inoculant improved fermentation quality, with significantly (P < 0.05) lower pH, ammonia nitrogen content, and gas production and higher lactic acid and acetic acid contents than those of the control. Enterobacteriaceae was the main bacterial community in early stage of fermentation, which utilizes sugar to produce CO2 gas, causing dry matter (DM) and energy loss. As fermentation progressed, the microbial diversity decreased, and the microbial community shifted from Gram-negative to Gram-positive bacteria. The inoculation of multispecies LAB displayed community synergy; Pediococcus acidilactici formed a dominant community in the early stage of fermentation, which produced an acid and anaerobic environment for the subsequent growth of Lentilactobacillus and Lacticaseibacillus species, thus forming a LAB-dominated microbial community. The predicted functional profile indicated that the silage inoculated with LI1 enhanced the carbohydrate metabolism pathway but inhibited the amino acid metabolism pathway, which played a role in promoting faster lactic acid production, reducing the decomposition of protein to ammonia nitrogen, and improving the fermentation quality of silage. Therefore, oat silage can be processed to high-quality and cleaner fermented feed by using an LAB inoculant, and LI1 showed better efficiency than LI2. IMPORTANCE Oat natural silage is rich in Enterobacteriaceae, increasing gas production and fermentation loss. Lactic acid bacteria interact synergistically to form a dominant community during ensiling. Pediococci grow vigorously in the early stage of fermentation and create an anaerobic environment. Lactobacilli inhibit the harmful microorganisms and result in cleaner fermentation of oat silage.

UI MeSH Term Description Entries
D009584 Nitrogen An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
D004755 Enterobacteriaceae A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock. Coliform Bacilli,Enterobacteria,Ewingella,Leclercia,Paracolobactrum,Sodalis
D005285 Fermentation Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID. Fermentations
D000641 Ammonia A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.
D012820 Silage Fodder converted into succulent feed for livestock through processes of anaerobic fermentation (as in a silo).
D056546 Lactobacillales An order of gram-positive bacteria in the class Bacilli, that have the ability to ferment sugars to lactic acid. They are widespread in nature and commonly used to produce FERMENTED FOODS. Lactic Acid Bacteria
D059827 Agricultural Inoculants Beneficial microorganisms (bacteria or fungi) encapsulated in carrier material and applied to the environment for remediation and enhancement of agricultural productivity. Microbial Inoculants,Bacterial Inoculants,Fungal Inoculants,Agricultural Inoculant,Bacterial Inoculant,Fungal Inoculant,Inoculant, Agricultural,Inoculant, Bacterial,Inoculant, Fungal,Inoculant, Microbial,Inoculants, Agricultural,Inoculants, Bacterial,Inoculants, Fungal,Inoculants, Microbial,Microbial Inoculant
D018554 Avena Oats, genus of the family POACEAE. Avena sativa,Oats,Cultivated Oat,Avenas,Cultivated Oats,Oat,Oat, Cultivated,Oats, Cultivated
D019344 Lactic Acid A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed) Lactate,2-Hydroxypropanoic Acid,2-Hydroxypropionic Acid,Ammonium Lactate,D-Lactic Acid,L-Lactic Acid,Propanoic Acid, 2-Hydroxy-, (2R)-,Propanoic Acid, 2-Hydroxy-, (2S)-,Sarcolactic Acid,2 Hydroxypropanoic Acid,2 Hydroxypropionic Acid,D Lactic Acid,L Lactic Acid,Lactate, Ammonium

Related Publications

Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Response of fermentation quality and microbial community of oat silage to homofermentative lactic acid bacteria inoculation.
January 2022, Frontiers in microbiology,
Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Microbial community and fermentation characteristic of Italian ryegrass silage prepared with corn stover and lactic acid bacteria.
May 2019, Bioresource technology,
Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Milk Production and Quality of Lactating Yak Fed Oat Silage Prepared with a Low-Temperature-Tolerant Lactic Acid Bacteria Inoculant.
October 2021, Foods (Basel, Switzerland),
Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Natural lactic acid bacteria population of tropical grasses and their fermentation factor analysis of silage prepared with cellulase and inoculant.
December 2016, Journal of dairy science,
Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production.
June 2021, Veterinary sciences,
Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Characteristics of lactic acid bacteria, microbial community and fermentation dynamics of native grass silage prepared in Inner Mongolian Plateau.
January 2022, Frontiers in microbiology,
Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Cellulase-lactic acid bacteria synergy action regulates silage fermentation of woody plant.
August 2023, Biotechnology for biofuels and bioproducts,
Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Microbial Community and FermentationDynamics of Corn Silage Prepared withHeat-Resistant Lactic Acid Bacteria in a HotEnvironment.
May 2020, Microorganisms,
Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Silage fermentation and ruminal degradation of stylo prepared with lactic acid bacteria and cellulase.
October 2017, Animal science journal = Nihon chikusan Gakkaiho,
Lin Sun, and Yanlin Xue, and Yanzi Xiao, and Rigele Te, and Xiaoguang Wu, and Na Na, and Nier Wu, and Moge Qili, and Yi Zhao, and Yimin Cai
Fermentation characteristics and microbial community composition of wet brewer's grains and corn stover mixed silage prepared with cellulase and lactic acid bacteria supplementation.
January 2024, Animal bioscience,
Copied contents to your clipboard!