BIOMAT Database Logo BIOMAT Database Logo

Effects of drought and rehydration on root gene expression in seedlings of Pinus massoniana Lamb. 2023

Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Road, Beijing 100091, China.

The mechanisms underlying plant response to drought involve the expression of numerous functional and regulatory genes. Transcriptome sequencing based on the second- and/or third-generation high-throughput sequencing platforms has proven to be powerful for investigating the transcriptional landscape under drought stress. However, the full-length transcriptomes related to drought responses in the important conifer genus Pinus L. remained to be delineated using the third-generation sequencing technology. With the objectives of identifying the candidate genes responsible for drought and/or rehydration and clarifying the expression profile of key genes involved in drought regulation, we combined the third- and second-generation sequencing techniques to perform transcriptome analysis on seedling roots under drought stress and rewatering in the drought-tolerant conifer Pinus massoniana Lamb. A sum of 294,114 unique full-length transcripts were produced with a mean length of 3217 bp and N50 estimate of 5075 bp, including 279,560 and 124,438 unique full-length transcripts being functionally annotated and Gene Ontology enriched, respectively. A total of 4076, 6295 and 18,093 differentially expressed genes (DEGs) were identified in three pair-wise comparisons of drought-treatment versus control transcriptomes, including 2703, 3576 and 8273 upregulated and 1373, 2719 and 9820 downregulated DEGs, respectively. Moreover, 157, 196 and 691 DEGs were identified as transcription factors in the three transcriptome comparisons and grouped into 26, 34 and 44 transcription factor families, respectively. Gene Ontology enrichment analysis revealed that a remarkable number of DEGs were enriched in soluble sugar-related and cell wall-related processes. A subset of 75, 68 and 97 DEGs were annotated to be associated with starch, sucrose and raffinose metabolism, respectively, while 32 and 70 DEGs were associated with suberin and lignin biosynthesis, respectively. Weighted gene co-expression network analysis revealed modules and hub genes closely related to drought and rehydration. This study provides novel insights into root transcriptomic changes in response to drought dynamics in Masson pine and serves as a fundamental work for further molecular investigation on drought tolerance in conifers.

UI MeSH Term Description Entries
D005440 Fluid Therapy Therapy whose basic objective is to restore the volume and composition of the body fluids to normal with respect to WATER-ELECTROLYTE BALANCE. Fluids may be administered intravenously, orally, by intermittent gavage, or by HYPODERMOCLYSIS. Oral Rehydration Therapy,Rehydration,Rehydration, Oral,Oral Rehydration,Rehydration Therapy, Oral,Therapy, Fluid,Therapy, Oral Rehydration,Fluid Therapies,Oral Rehydration Therapies,Oral Rehydrations,Rehydration Therapies, Oral,Rehydrations,Rehydrations, Oral,Therapies, Fluid,Therapies, Oral Rehydration
D013312 Stress, Physiological The unfavorable effect of environmental factors (stressors) on the physiological functions of an organism. Prolonged unresolved physiological stress can affect HOMEOSTASIS of the organism, and may lead to damaging or pathological conditions. Biotic Stress,Metabolic Stress,Physiological Stress,Abiotic Stress,Abiotic Stress Reaction,Abiotic Stress Response,Biological Stress,Metabolic Stress Response,Physiological Stress Reaction,Physiological Stress Reactivity,Physiological Stress Response,Abiotic Stress Reactions,Abiotic Stress Responses,Abiotic Stresses,Biological Stresses,Biotic Stresses,Metabolic Stress Responses,Metabolic Stresses,Physiological Stress Reactions,Physiological Stress Responses,Physiological Stresses,Reaction, Abiotic Stress,Reactions, Abiotic Stress,Response, Abiotic Stress,Response, Metabolic Stress,Stress Reaction, Physiological,Stress Response, Metabolic,Stress Response, Physiological,Stress, Abiotic,Stress, Biological,Stress, Biotic,Stress, Metabolic
D014157 Transcription Factors Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. Transcription Factor,Factor, Transcription,Factors, Transcription
D055864 Droughts Prolonged dry periods in natural climate cycle. They are slow-onset phenomena caused by rainfall deficit combined with other predisposing factors. Drought
D059467 Transcriptome The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells. Transcriptomes,Gene Expression Profiles,Gene Expression Signatures,Transcriptome Profiles,Expression Profile, Gene,Expression Profiles, Gene,Expression Signature, Gene,Expression Signatures, Gene,Gene Expression Profile,Gene Expression Signature,Profile, Gene Expression,Profile, Transcriptome,Profiles, Gene Expression,Profiles, Transcriptome,Signature, Gene Expression,Signatures, Gene Expression,Transcriptome Profile
D018506 Gene Expression Regulation, Plant Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants. Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant
D020869 Gene Expression Profiling The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell. Gene Expression Analysis,Gene Expression Pattern Analysis,Transcript Expression Analysis,Transcriptome Profiling,Transcriptomics,mRNA Differential Display,Gene Expression Monitoring,Transcriptome Analysis,Analyses, Gene Expression,Analyses, Transcript Expression,Analyses, Transcriptome,Analysis, Gene Expression,Analysis, Transcript Expression,Analysis, Transcriptome,Differential Display, mRNA,Differential Displays, mRNA,Expression Analyses, Gene,Expression Analysis, Gene,Gene Expression Analyses,Gene Expression Monitorings,Gene Expression Profilings,Monitoring, Gene Expression,Monitorings, Gene Expression,Profiling, Gene Expression,Profiling, Transcriptome,Profilings, Gene Expression,Profilings, Transcriptome,Transcript Expression Analyses,Transcriptome Analyses,Transcriptome Profilings,mRNA Differential Displays
D028223 Pinus A plant genus in the family PINACEAE, order Pinales, class Pinopsida, division Coniferophyta. They are evergreen trees mainly in temperate climates. Pine Tree,Maritime Pine,Pinus abies,Pinus maritima,Pinus pinaster,Pinus radiata,Pinus tremula,Maritime Pines,Pine Trees,Pine, Maritime,Pines, Maritime,Tree, Pine,Trees, Pine
D036226 Seedlings Young plants produced at the completion of GERMINATION of SEEDS. Seedling,Malted Grain,Sprouted Seeds,Sprouts, Plant,Grain, Malted,Grains, Malted,Malted Grains,Plant Sprout,Plant Sprouts,Seed, Sprouted,Seeds, Sprouted,Sprout, Plant,Sprouted Seed

Related Publications

Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
Effect of Ectomycorrhizal Fungi on the Drought Resistance of Pinus massoniana Seedlings.
April 2023, Journal of fungi (Basel, Switzerland),
Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
[Physiological responses of mycorrhizal Pinus massoniana seedlings to drought stress and drought resistance evaluation].
March 2013, Ying yong sheng tai xue bao = The journal of applied ecology,
Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
[Studies on the physiological behavior of Pinus merkusii Jungh. and Pinus massoniana Lamb].
January 1976, Beitrage zur tropischen Landwirtschaft und Veterinarmedizin,
Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
Structure and chlorophyll fluorescence of heteroblastic foliage affect first-year growth in Pinus massoniana Lamb. seedlings.
January 2022, Plant physiology and biochemistry : PPB,
Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
Effects of exogenous GA3 on stem secondary growth of Pinus massoniana seedlings.
January 2024, Plant physiology and biochemistry : PPB,
Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
Effects of PmaIAA27 and PmaARF15 genes on drought stress tolerance in pinus massoniana.
October 2023, BMC plant biology,
Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
Correction: Effects of PmaIAA27 and PmaARF15 genes on drought stress tolerance in pinus massoniana.
October 2023, BMC plant biology,
Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
Transcriptome Analysis of Needle and Root of Pinus Massoniana in Response to Continuous Drought Stress.
April 2021, Plants (Basel, Switzerland),
Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
Soil microbial residue characteristics in Pinus massoniana lamb. Plantations.
August 2023, Environmental research,
Xinhua Chen, and Hu Chen, and Huilan Xu, and Mei Li, and Qunfeng Luo, and Ting Wang, and Zhangqi Yang, and Siming Gan
Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings.
July 2021, International journal of molecular sciences,
Copied contents to your clipboard!