Radiofrequency emitted radiations (RFEMR) from mobile phones are known to produce a stress response because of its effect on hypothalamus. Mobile phones have become an integral part of our lives with increasing usage not only in terms of number of users but also increase in talk time. Therefore, it is of interest to study the effect of mobile phone radiofrequency electromagnetic radiations on NSE and MDA levels in SD rats. Twelve male SD rats of 10-12weeks old, weighing 180-220 grams, were purchased from registered laboratory breeders & housed in a room with 12:12hour's light-dark cycle with adlibitum amount of food and RO water. Present study showed significant increase in NSE and MDA levels in rats exposed to RFEMR. This study proves that mobile RFEMR causes oxidative stress and oxidative damage in SD rats.