BIOMAT Database Logo BIOMAT Database Logo

500-MHz 1H NMR study of poly(dG).poly(dC) in solution using one-dimensional nuclear Overhauser effect. 1986

M H Sarma, and G Gupta, and R H Sarma

Secondary structures of poly(dG).poly(dC) and poly(dG).poly(dm5C) in solution are determined by nuclear Overhauser effect (NOE) measurements on GH8-deuterated and -nondeuterated DNAs with low presaturation pulse lengths (10-25 ms) and low-power and prolonged accumulations in the range of 50,000-72,000 scans. Under these conditions, the NOE difference spectra were free from diffusion. Primary NOEs between base protons GH8/CH6 and sugar protons H1', H2'/H2'', and H3' suggest that in poly(dG).poly(dC) both guanine and cytosine nucleotides adopt a C3'-endo, low anti X = 200-220 degrees conformation. Computer modeling of the NOE data enable identification for the first time, in terms of the geometry of the nucleotide repeat, handedness, and helix geometry, of the structure of poly(dG).poly(dC) to be the A form, and the derived structure for the polymer duplex is very close to the single crystal structure of the double-helical d-GGGGCCCC [McCall, M., Brown, T., & Kennard, O. (1985) J. Mol. Biol. 183, 385-396]. Similar nuclear Overhauser effect data on poly(dG).poly(dm5C) revealed that G and m5C adopt a C2'endo, anti X = 240-260 degrees conformation, which indicates that this DNA exhibits the B form in solution. In summary, the results presented in this paper demonstrate that methylation of cytosines in poly(dG).poly(dC) causes A----B transition in the molecule.

UI MeSH Term Description Entries
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D009682 Magnetic Resonance Spectroscopy Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING). In Vivo NMR Spectroscopy,MR Spectroscopy,Magnetic Resonance,NMR Spectroscopy,NMR Spectroscopy, In Vivo,Nuclear Magnetic Resonance,Spectroscopy, Magnetic Resonance,Spectroscopy, NMR,Spectroscopy, Nuclear Magnetic Resonance,Magnetic Resonance Spectroscopies,Magnetic Resonance, Nuclear,NMR Spectroscopies,Resonance Spectroscopy, Magnetic,Resonance, Magnetic,Resonance, Nuclear Magnetic,Spectroscopies, NMR,Spectroscopy, MR
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D011089 Polydeoxyribonucleotides A group of 13 or more deoxyribonucleotides in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties. Polydeoxyribonucleotide
D006859 Hydrogen The first chemical element in the periodic table with atomic symbol H, and atomic number 1. Protium (atomic weight 1) is by far the most common hydrogen isotope. Hydrogen also exists as the stable isotope DEUTERIUM (atomic weight 2) and the radioactive isotope TRITIUM (atomic weight 3). Hydrogen forms into a diatomic molecule at room temperature and appears as a highly flammable colorless and odorless gas. Protium,Hydrogen-1
D012996 Solutions The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed) Solution

Related Publications

M H Sarma, and G Gupta, and R H Sarma
Internal motions in B- and Z-form poly(dG-dC).poly(dG-dC): 1H NMR relaxation studies.
October 1985, Biochemistry,
M H Sarma, and G Gupta, and R H Sarma
Structure of Alamethicin in solution. One- and two-dimensional 1H nuclear magnetic resonance studies at 500 MHz.
April 1983, Journal of molecular biology,
M H Sarma, and G Gupta, and R H Sarma
Alkaline titrations of poly(dG-dC).poly(dG-dC): microemulsion versus solution behavior.
June 2007, Journal of biomolecular structure & dynamics,
M H Sarma, and G Gupta, and R H Sarma
1H two-dimensional nuclear Overhauser effect and relaxation studies of poly(dA).poly(dT)
June 1986, Biochemistry,
M H Sarma, and G Gupta, and R H Sarma
Comparison of poly(dG-dC).poly(dG-dC) conformations in oriented films and in solution.
January 1982, Proceedings of the National Academy of Sciences of the United States of America,
M H Sarma, and G Gupta, and R H Sarma
Solution conformation of the biantennary N-linked oligosaccharide of human serotransferrin using 1H NMR nuclear Overhauser effect measurements.
December 1982, FEBS letters,
M H Sarma, and G Gupta, and R H Sarma
Acid titrations of poly(dG-dC).poly(dG-dC) in aqueous solution and in a w/o microemulsion.
February 2006, Journal of biomolecular structure & dynamics,
M H Sarma, and G Gupta, and R H Sarma
An A-form poly(dG).poly(dC) in H2O solution.
September 1985, Biopolymers,
M H Sarma, and G Gupta, and R H Sarma
A 500 MHz 1H NMR spectroscopic study of Met5-enkephalinamide in aqueous solution: ethanol induced conformational changes.
February 1987, Biochemical and biophysical research communications,
M H Sarma, and G Gupta, and R H Sarma
Analysis of membrane lipids by 500 MHz 1H NMR.
April 1989, Analytical biochemistry,
Copied contents to your clipboard!