BIOMAT Database Logo BIOMAT Database Logo

Chick retinal pigment epithelium exhibits glutathione requiring prostaglandin D2 synthetase activity. 1986

R Yamakawa, and N Ogino

Prostaglandin synthetic activity of neural retina and retinal pigment epithelium in chick retina was studied using arachidonic acid and prostaglandin H2 (intermediate prostaglandin endoperoxide) as substrates. To obtain sufficient enzyme activity in retinal pigment epithelium, cultured chick retinal pigment epithelial cells were used. Little arachidonic acid was converted into prostaglandins, and details of prostaglandin synthetic activity were not clear. The assay from prostaglandin H2 showed that: 1) preparations consisting of both neural retina and retinal pigment epithelium dissected from 7-day-old chicks have activity of glutathione-requiring prostaglandin D2 synthetase; 2) preparations consisting of only neural retina dissected from 7-day-old chicks have no activity of prostaglandin synthetase; and 3) cultured chick retinal pigment epithelial cells have high activity of glutathione-requiring prostaglandin D2 synthetase.

UI MeSH Term Description Entries
D007535 Isomerases A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5. Isomerase
D010857 Pigment Epithelium of Eye The layer of pigment-containing epithelial cells in the RETINA; the CILIARY BODY; and the IRIS in the eye. Eye Pigment Epithelium
D011449 Prostaglandin Endoperoxides Precursors in the biosynthesis of prostaglandins and thromboxanes from arachidonic acid. They are physiologically active compounds, having effect on vascular and airway smooth muscles, platelet aggregation, etc. Endoperoxides, Prostaglandin
D011450 Prostaglandin Endoperoxides, Synthetic Synthetic compounds that are analogs of the naturally occurring prostaglandin endoperoxides and that mimic their pharmacologic and physiologic activities. They are usually more stable than the naturally occurring compounds. Prostaglandin Endoperoxide Analogs,Prostaglandin Endoperoxide Analogues,Synthetic Prostaglandin Endoperoxides,Analogues, Prostaglandin Endoperoxide,Endoperoxide Analogues, Prostaglandin,Endoperoxides, Synthetic Prostaglandin
D011453 Prostaglandins A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes. Prostaglandin,Prostanoid,Prostanoids
D011463 Prostaglandins H A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. The most frequently encountered member of this group is the prostaglandin H2.
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D005978 Glutathione A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides. Reduced Glutathione,gamma-L-Glu-L-Cys-Gly,gamma-L-Glutamyl-L-Cysteinylglycine,Glutathione, Reduced,gamma L Glu L Cys Gly,gamma L Glutamyl L Cysteinylglycine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D044262 Prostaglandin H2 A cyclic endoperoxide intermediate produced by the action of CYCLOOXYGENASE on ARACHIDONIC ACID. It is further converted by a series of specific enzymes to the series 2 prostaglandins. PGH2,PGH(2)

Related Publications

R Yamakawa, and N Ogino
[Production and metabolism of prostaglandin D2 by cultured chick retinal pigment epithelial cells].
July 1996, Nippon Ganka Gakkai zasshi,
R Yamakawa, and N Ogino
Tyrosinase from embryonic chick retinal pigment epithelium.
November 1973, Comparative biochemistry and physiology. B, Comparative biochemistry,
R Yamakawa, and N Ogino
Melanogenesis of retinal pigment epithelium of chick embryo.
January 1981, Journal of electron microscopy,
R Yamakawa, and N Ogino
Identification of GABA receptors in chick retinal pigment epithelium.
February 2013, Neuroscience letters,
R Yamakawa, and N Ogino
Myeloid body development in the chick retinal pigment epithelium.
February 1992, Current eye research,
R Yamakawa, and N Ogino
Effects of current clamp on chick retinal pigment epithelium.
June 1991, Investigative ophthalmology & visual science,
R Yamakawa, and N Ogino
Characterization of calcium uptake in chick retinal pigment epithelium.
September 1990, Pigment cell research,
R Yamakawa, and N Ogino
Chick retinal pigment epithelium transdifferentiation assay for proneural activities.
January 2012, Methods in molecular biology (Clifton, N.J.),
R Yamakawa, and N Ogino
Retinal pigment epithelium exhibits gene expression and phagocytic activity alterations when exposed to retinoblastoma chemotherapeutics.
August 2023, Experimental eye research,
R Yamakawa, and N Ogino
Synthesis of glycosaminoglycans by chick retinal pigment epithelium in vitro.
January 1984, Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
Copied contents to your clipboard!