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Serial Block Face-Scanning Electron Microscopy as a Burgeoning Technology. 2023
Andrea G Marshall, and
Kit Neikirk, and
Dominique C Stephens, and
Larry Vang, and
Zer Vue, and
Heather K Beasley, and
Amber Crabtree, and
Estevão Scudese, and
Edgar Garza Lopez, and
Bryanna Shao, and
Evan Krystofiak, and
Sharifa Rutledge, and
Jaimaine Davis, and
Sandra A Murray, and
Steven M Damo, and
Prasanna Katti, and
Antentor Hinton
Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Serial block face scanning electron microscopy (SBF-SEM), also referred to as serial block-face electron microscopy, is an advanced ultrastructural imaging technique that enables three-dimensional visualization that provides largerx- and y-axis ranges than other volumetric EM techniques. While SEM is first introduced in the 1930s, SBF-SEM is developed as a novel method to resolve the 3D architecture of neuronal networks across large volumes with nanometer resolution by Denk and Horstmann in 2004. Here, the authors provide an accessible overview of the advantages and challenges associated with SBF-SEM. Beyond this, the applications of SBF-SEM in biochemical domains as well as potential future clinical applications are briefly reviewed. Finally, the alternative forms of artificial intelligence-based segmentation which may contribute to devising a feasible workflow involving SBF-SEM, are also considered.
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