Genetic injury in hybrid male mice exposed to low doses of 60Co gamma-rays or fission neutrons. II. Dominant lethal mutation response to long-term weekly exposures. 1986

D Grahn, and B A Carnes, and B H Farrington

Male B6CF1 mice were exposed to once-weekly doses of either fission neutrons or 60Co gamma-rays for periods up to one year and mated periodically to screen for the induction of dominant lethal mutations. Two independent experiments were performed, each involving a control and three dose levels of both neutrons and gamma-rays. Neutron doses were between 0.125 and 2.67 rad/week and gamma-ray levels were between 5 and 32 rad/week. Data on both pre- and post-implantation fetal deaths were obtained. Analyses that were intended to identify the potential contribution from age- or time-dependent factors, which could include changes in radiosensitivity and in spontaneous rates plus any cumulative damage to the stem cell population did not reveal a consistent significant contribution to the mutation rate/rad/week. Direct comparisons of these data with data from males exposed to single doses confirm that weekly neutron irradiation is significantly more effective than single doses for the induction of postimplant fetal losses, whereas single doses of gamma-rays are more effective than the same dose divided into weekly fractions. Neutron-induced augmentation appears limited in these data to lethal mutations induced in meiotic and postmeiotic cell stages. The relative biological effectiveness (RBE) of neutrons rises from 5 +/- 1 to 12 +/- 1 for single vs. weekly doses. Rates of preimplant loss, although significant, are not a sensitive measure of genetic injury at the low doses used here. They are extremely sensitive to litter size and best estimated in litters of seven or more implants along with appropriate statistical control of concurrent variation in the number of corpora lutea.

UI MeSH Term Description Entries
D008297 Male Males
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D009502 Neutrons Electrically neutral elementary particles found in all atomic nuclei except light hydrogen; the mass is equal to that of the proton and electron combined and they are unstable when isolated from the nucleus, undergoing beta decay. Slow, thermal, epithermal, and fast neutrons refer to the energy levels with which the neutrons are ejected from heavier nuclei during their decay. Neutron
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D011833 Radiation Injuries, Experimental Experimentally produced harmful effects of ionizing or non-ionizing RADIATION in CHORDATA animals. Experimental Radiation Injuries,Injuries, Experimental Radiation,Experimental Radiation Injury,Radiation Injury, Experimental
D003037 Cobalt Radioisotopes Unstable isotopes of cobalt that decay or disintegrate emitting radiation. Co atoms with atomic weights of 54-64, except 59, are radioactive cobalt isotopes. Radioisotopes, Cobalt
D004307 Dose-Response Relationship, Radiation The relationship between the dose of administered radiation and the response of the organism or tissue to the radiation. Dose Response Relationship, Radiation,Dose-Response Relationships, Radiation,Radiation Dose-Response Relationship,Radiation Dose-Response Relationships,Relationship, Radiation Dose-Response,Relationships, Radiation Dose-Response
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005260 Female Females
D005799 Genes, Dominant Genes that influence the PHENOTYPE both in the homozygous and the heterozygous state. Conditions, Dominant Genetic,Dominant Genetic Conditions,Genetic Conditions, Dominant,Condition, Dominant Genetic,Dominant Gene,Dominant Genes,Dominant Genetic Condition,Gene, Dominant,Genetic Condition, Dominant

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