-transparent.png){kind=logo}
A bioautographic assay was developed for the visualization of aminoacylase-1 (N-acylamino acid aminohydrolase, ACY-1; EC 3.5.1.14) after zone electrophoresis. Bioautography and species differences in electrophoretic mobility of ACY-1 made it possible to investigate the chromosome assignment of the gene for human ACY-1 using human--mouse somatic cell hybrids. Human ACY-1 segregated concordantly with beta-galactosidase-A (beta GALA; EC 3.2.1.23) but showed discordant segregation with 32 other markers representing 23 linkage groups. The beta GALA gene has been previously assigned to chromosome 3. From this evidence and confirming chromosome analyses, ACY-1 has been assigned to chromosome 3. A genetic polymorphism in the electrophoretic mobility of ACY was observed in mouse strains, demonstrating that this enzyme can be mapped in genetic crosses of Mus musculus.
S L Naylor, and
T B Shows, and
R J Klebe
July 1980,
Annals of human genetics,
S L Naylor, and
T B Shows, and
R J Klebe
August 1980,
Biochemical genetics,
S L Naylor, and
T B Shows, and
R J Klebe
January 1978,
Cytogenetics and cell genetics,
S L Naylor, and
T B Shows, and
R J Klebe
September 1988,
Somatic cell and molecular genetics,
S L Naylor, and
T B Shows, and
R J Klebe
September 1974,
Proceedings of the National Academy of Sciences of the United States of America,
S L Naylor, and
T B Shows, and
R J Klebe
July 1986,
Investigative ophthalmology & visual science,
S L Naylor, and
T B Shows, and
R J Klebe
January 1986,
Cytogenetics and cell genetics,
S L Naylor, and
T B Shows, and
R J Klebe
January 1978,
Cytogenetics and cell genetics,
S L Naylor, and
T B Shows, and
R J Klebe
September 1990,
Genomics,
S L Naylor, and
T B Shows, and
R J Klebe
January 1974,
Cytogenetics and cell genetics,
