-transparent.png){kind=logo}
We have developed an in vitro method for studying ethanol-induced injury to gastric mucosa using organ culture of rat antrum. Cell damage was assessed by measurement of the release of [51Cr]sodium chromate from preloaded cells, a method adapted from a standard immunologic technique. This system provided rapid and highly reproducible quantitation of tissue injury as assessed by 51Cr release into the culture medium. The threshold concentration for ethanol-induced damage was between 10 and 15% v/v, similar to in vivo thresholds observed by others. 51Cr release could also be induced by very short exposure to ethanol (5-15 min), and then continued despite ethanol removal. Interestingly, after continuous ethanol exposure, a plateau of maximum 51Cr release was reached 60 min after exposure to ethanol over the concentration range 20-50%, suggesting tissue adaptation to ethanol damage. This organ culture system, which allows precise control of experimental conditions, may be useful for studying mechanisms of gastric mucosal injury and protection.
R B Sewell, and
T S Ling, and
N D Yeomans
November 1983,
The Journal of clinical investigation,
R B Sewell, and
T S Ling, and
N D Yeomans
September 1985,
Digestive diseases and sciences,
R B Sewell, and
T S Ling, and
N D Yeomans
January 1997,
Neurotoxicology and teratology,
R B Sewell, and
T S Ling, and
N D Yeomans
January 1986,
Digestion,
R B Sewell, and
T S Ling, and
N D Yeomans
January 1983,
Biomedica biochimica acta,
R B Sewell, and
T S Ling, and
N D Yeomans
January 1987,
Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement,
R B Sewell, and
T S Ling, and
N D Yeomans
March 1976,
Sangyo igaku. Japanese journal of industrial health,
R B Sewell, and
T S Ling, and
N D Yeomans
September 1994,
Environmental health perspectives,
R B Sewell, and
T S Ling, and
N D Yeomans
March 1987,
Regulatory peptides,
R B Sewell, and
T S Ling, and
N D Yeomans
August 1987,
Agents and actions,
