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Specificity of naturally occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus: determination by an enzyme linked immunosorbent assay. 1986

M Tauchi, and Y Kanai, and H Hashimoto, and S Hirose

Serum autoantibodies to poly(ADP-ribose), single-stranded (ss) DNA and double-stranded (ds) DNA in 145 patients with systemic lupus erythematosus (SLE) were measured by an enzyme-linked immunosorbent assay (ELISA). The specificities of the antibodies for poly(ADP-ribose) or for ssDNA in 14 serum samples from different patients, who had relatively high antibody titers to either or both these antigens, were tested by competitive ELISA with poly(ADP-ribose) and ssDNA as inhibitors. The IgG class anti-poly(ADP-ribose) antibodies of 4 serum samples (cases 9, 11, 13 and 14) preferred poly(ADP-ribose) and those of 2 samples (cases 2 and 4) cross-reacted preferentially with ssDNA, while the IgG class anti-ssDNA antibodies of 2 serum samples (cases 9 and 11) significantly cross-reacted with poly(ADP-ribose). Hence, the nature of the antibodies to poly(ADP-ribose) in SLE patients seemed to be different from that of the anti-poly(ADP-ribose) antibodies in autoimmune MRL/Mp-lpr/lpr (MRL/l) mice, which seem to be subpopulations of anti-ssDNA antibodies and react equally well with poly(ADP-ribose) and ssDNA.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007075 Immunoglobulin M A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin. Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D008180 Lupus Erythematosus, Systemic A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. Libman-Sacks Disease,Lupus Erythematosus Disseminatus,Systemic Lupus Erythematosus,Disease, Libman-Sacks,Libman Sacks Disease
D008297 Male Males
D009702 Nucleoside Diphosphate Sugars Diphosphate Sugars, Nucleoside,Sugars, Nucleoside Diphosphate
D011064 Poly Adenosine Diphosphate Ribose A polynucleotide formed from the ADP-RIBOSE moiety of nicotinamide-adenine dinucleotide (NAD) by POLY(ADP-RIBOSE) POLYMERASES. Poly ADP Ribose,Poly(ADP-Ribose),Poly-ADPR,Poly-Adenosine Diphosphate-Ribose,ADP Ribose, Poly,Diphosphate-Ribose, Poly-Adenosine,Poly ADPR,Ribose, Poly ADP
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004277 DNA, Single-Stranded A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle. Single-Stranded DNA,DNA, Single Stranded,Single Stranded DNA
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females

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