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Perfusion fixation is essential for the examination of the hepatic microvasculature by scanning electron microscopy. No detailed study has previously been done on the perfusion of human hepatic needle biopsies. The present paper demonstrates two modifications of a simple technique by which needle biopsies can be perfused. Perfusion was performed either manually (n = 33) or with a constant perfusion pressure of 10-15 mm H2O (n = 54). No significant difference in the ultrastructural preservation was observed in the two groups. Manual perfusion, however, when done gently, was superior in cleaning the sinusoids, and thus larger areas could be investigated by this method. Topographical orientation was possible, allowing the identification of terminal hepatic veins and portal tracts.
T Horn, and
P Christoffersen
May 1977,
Acta pathologica et microbiologica Scandinavica. Section A, Pathology,
T Horn, and
P Christoffersen
January 1990,
Diagnostic cytopathology,
T Horn, and
P Christoffersen
January 1980,
Scanning electron microscopy,
T Horn, and
P Christoffersen
February 1976,
Gastrointestinal endoscopy,
T Horn, and
P Christoffersen
January 1980,
Artery,
T Horn, and
P Christoffersen
May 2020,
Applied microscopy,
T Horn, and
P Christoffersen
August 1975,
Experientia,
T Horn, and
P Christoffersen
May 1965,
The Journal of the American Osteopathic Association,
T Horn, and
P Christoffersen
January 1996,
Ultrastructural pathology,
T Horn, and
P Christoffersen
January 1977,
Acta cytologica,
