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The streptozotocin diabetic rat was selected as a model to study how insulin deficiency alters vitamin B6 utilization by focusing on pyridoxal phosphate levels and aspartate aminotransferase activities in liver tissues. Diabetes of 15 weeks' duration lowered plasma pyridoxal phosphate levels by 84%. Normal plasma pyridoxal phosphate was 480 pmole/ml. Fractionation of liver into mitochondrial and extramitochondrial compartments demonstrated that diabetes caused a 43% diminution in mitochondrial pyridoxal phosphate per gram of liver. There was no cytoplasmic change in these diabetic rats. Mitochondrial aspartate aminotransferase activity was decreased 53% per gram of diabetic liver and cytoplasmic aspartate aminotransferase activity was elevated 3.4-fold. Damage to diabetic mitochondria during preparation procedures could not account for the rise in cytoplasmic aspartate aminotransferase activity. Electrophoresis showed that in the diabetic cytoplasm both cathodal and anodal forms of the enzyme were elevated. Speculations concerning mitochondrial loss and cytoplasmic gain of enzyme activity as well as those on the reduction of plasma pyridoxal phosphate in the diabetic rat are presented.
K S Rogers, and
E S Higgins, and
E S Kline
April 2003,
Biochimica et biophysica acta,
K S Rogers, and
E S Higgins, and
E S Kline
May 1980,
Proceedings of the National Academy of Sciences of the United States of America,
K S Rogers, and
E S Higgins, and
E S Kline
March 1979,
Archives of biochemistry and biophysics,
K S Rogers, and
E S Higgins, and
E S Kline
June 1978,
Journal of neurochemistry,
K S Rogers, and
E S Higgins, and
E S Kline
May 1984,
FEBS letters,
K S Rogers, and
E S Higgins, and
E S Kline
November 1986,
The Journal of laboratory and clinical medicine,
K S Rogers, and
E S Higgins, and
E S Kline
December 2010,
Journal of the American Chemical Society,
K S Rogers, and
E S Higgins, and
E S Kline
January 1973,
Journal of nutritional science and vitaminology,
K S Rogers, and
E S Higgins, and
E S Kline
January 1973,
Clinical chemistry,
K S Rogers, and
E S Higgins, and
E S Kline
November 1982,
Annals of clinical biochemistry,
