Biomaterial Database

A comparative study of protoplast preparation and regeneration in Streptomyces rimosus and Streptomyces lividans. 1986

D Hranueli, and T Smokvina, and M Alacević

One of the basic techniques for DNA cloning in Streptomyces is the preparation of protoplasts and the efficient regeneration of normal mycelia. In order to develop an oxytetracycline producing S. rimosus strain as a host for molecular cloning, the efficiencies of protoplast preparation and cell wall regeneration were compared with those obtained using S. lividans, the host for the majority of cloning experiments. The results presented suggest that the S. rimosus strain selected is a convenient host for the cloning of recombinant DNA, at least in relation to the preparation and regeneration of protoplasts. Interestingly, the size of the protoplasts was dependent upon the physiological age of the mycelium in S. rimosus; no such dependence was observed in S. lividans.

UI	MeSH Term	Description	Entries
D010118	Oxytetracycline	A TETRACYCLINE analog isolated from the actinomycete STREPTOMYCES RIMOSUS and used in a wide variety of clinical conditions.	Hydroxytetracycline,Bisolvomycin,Geomycin,Oxyterracin,Oxyterracine,Oxytetracid,Oxytetracycline Anhydrous,Oxytetracycline Calcium,Oxytetracycline Dihydrate,Oxytetracycline Hydrochloride,Oxytetracycline Monohydrochloride,Oxytetracycline Sulfate (2:1),Oxytetracycline, (4a beta,5 beta,5a beta,12a beta)-Isomer,Oxytetracycline, (5 beta)-Isomer,Oxytetracycline, Anhydrous,Oxytetracycline, Calcium (1:1) Salt,Oxytetracycline, Disodium Salt, Dihydrate,Oxytetracycline, Sodium Salt,Terramycin
D011523	Protoplasts	The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.	Protoplast
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D004274	DNA, Recombinant	Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.	Genes, Spliced,Recombinant DNA,Spliced Gene,Recombinant DNA Research,Recombination Joint,DNA Research, Recombinant,Gene, Spliced,Joint, Recombination,Research, Recombinant DNA,Spliced Genes
D013302	Streptomyces	A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.