Biomaterial Database

LRRC10 regulates mammalian cardiomyocyte cell cycle during heart regeneration. 2023

Rebecca J Salamon, and Megan C McKeon, and Jiyoung Bae, and Xiaoya Zhang, and Wyatt G Paltzer, and Kayla N Wanless, and Alyssa R Schuett, and Dakota J Nuttall, and Stephen A Nemr, and Rupa Sridharan, and Youngsook Lee, and Timothy J Kamp, and Ahmed I Mahmoud

Department of Cell and Regenerative Biology, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, 53705, USA.

Leucine-rich repeat containing 10 (LRRC10) is a cardiomyocyte-specific protein, but its role in cardiac biology is little understood. Recently Lrrc10 was identified as required for endogenous cardiac regeneration in zebrafish; however, whether LRRC10 plays a role in mammalian heart regeneration remains unclear. In this study, we demonstrate that Lrrc10-/- knockout mice exhibit a loss of the neonatal mouse regenerative response, marked by reduced cardiomyocyte cytokinesis and increased cardiomyocyte binucleation. Interestingly, LRRC10 deletion disrupts the regenerative transcriptional landscape of the regenerating neonatal mouse heart. Remarkably, cardiac overexpression of LRRC10 restores cardiomyocyte cytokinesis, increases cardiomyocyte mononucleation, and the cardiac regenerative capacity of Lrrc10-/- mice. Our results are consistent with a model in which LRRC10 is required for cardiomyocyte cytokinesis as well as regulation of the transcriptional landscape during mammalian heart regeneration.