Biomaterial Database

Acylation of 1-alkenyl-glycerophosphocholine and 1-acyl-glycerophosphocholine in guinea pig heart. 1986

G Arthur, and P C Choy

The deacylation-reacylation process has been shown to be an important pathway for phospholipids to attain the desired acyl groups at the C-2 position. The acylation of 1-acyl-glycerophosphocholine (-GPC) in mammalian hearts has been well documented, but the acylation of 1-alkenyl-GPC has not been described. In this paper, we demonstrate the presence of acyl-CoA: 1-alkenyl-GPC acyltransferase for the acylation of 1-alkenyl-GPC in mammalian hearts; the highest activity is found in guinea pig heart. The guinea pig heart 1-alkenyl-GPC acyltransferase has only 10-40% of the 1-acyl-GPC acyltransferase activity, and both activities are located in the microsomal fraction. However, these two enzymes respond differently to cations, detergents and heat treatment, and the two enzymes also display different acyl specificity. Kinetic studies indicate that both reactions could not be accommodated by the same catalytic site. The results provide strong evidence that the two activities are from separate and distinct proteins. The specificity of 1-alkenyl-GPC acyltransferase for unsaturated species of acyl-CoA may play an important role in the maintenance of the high degree of unsaturated acyl groups found in guinea pig heart plasmalogens.

UI	MeSH Term	Description	Entries
D008243	1-Acylglycerophosphocholine O-Acyltransferase	An enzyme localized predominantly within the plasma membrane of lymphocytes. It catalyzes the transfer of long-chain fatty acids, preferentially unsaturated fatty acids, to lysophosphatides with the formation of 1,2-diacylglycero-3-phosphocholine and CoA. EC 2.3.1.23.	1-Acylglycerophosphocholine Acyltransferase,Acyl CoA Lysolecithin Acyltransferase,Lysolecithin Acyltransferase,Acyl-CoA-1-Acylglycero-3-Phosphocholine-O-Acyltransferase,Lysophosphatidylcholine Acyltransferase,Lysophosphatidylcholine-Palmitoyl CoA Acyltransferase,Lysophospholipid Acyltransferase,1 Acylglycerophosphocholine Acyltransferase,1 Acylglycerophosphocholine O Acyltransferase,Acyl CoA 1 Acylglycero 3 Phosphocholine O Acyltransferase,Acyltransferase, 1-Acylglycerophosphocholine,Acyltransferase, Lysolecithin,Acyltransferase, Lysophosphatidylcholine,Acyltransferase, Lysophosphatidylcholine-Palmitoyl CoA,Acyltransferase, Lysophospholipid,CoA Acyltransferase, Lysophosphatidylcholine-Palmitoyl,Lysophosphatidylcholine Palmitoyl CoA Acyltransferase,O-Acyltransferase, 1-Acylglycerophosphocholine
D008244	Lysophosphatidylcholines	Derivatives of PHOSPHATIDYLCHOLINES obtained by their partial hydrolysis which removes one of the fatty acid moieties.	Lysolecithin,Lysolecithins,Lysophosphatidylcholine
D008274	Magnesium	A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
D008297	Male		Males
D009206	Myocardium	The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.	Muscle, Cardiac,Muscle, Heart,Cardiac Muscle,Myocardia,Cardiac Muscles,Heart Muscle,Heart Muscles,Muscles, Cardiac,Muscles, Heart
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D003902	Detergents	Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.	Cleansing Agents,Detergent Pods,Laundry Detergent Pods,Laundry Pods,Syndet,Synthetic Detergent,Agent, Cleansing,Agents, Cleansing,Cleansing Agent,Detergent,Detergent Pod,Detergent Pod, Laundry,Detergent Pods, Laundry,Detergent, Synthetic,Detergents, Synthetic,Laundry Detergent Pod,Laundry Pod,Pod, Detergent,Pod, Laundry,Pod, Laundry Detergent,Pods, Detergent,Pods, Laundry,Pods, Laundry Detergent,Synthetic Detergents
D005997	Glycerylphosphorylcholine	A component of PHOSPHATIDYLCHOLINES or LECITHINS, in which the two hydroxy groups of GLYCEROL are esterified with fatty acids. (From Stedman, 26th ed)	Choline Glycerophosphate,Choline Alfoscerate,Choline Alphoscerate,Glycerol 3-Phosphocholine,Glycerophosphorylcholine,L-alpha-Glycerylphosphorylcholine,3-Phosphocholine, Glycerol,Alfoscerate, Choline,Alphoscerate, Choline,Glycerol 3 Phosphocholine,Glycerophosphate, Choline,L alpha Glycerylphosphorylcholine
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006321	Heart	The hollow, muscular organ that maintains the circulation of the blood.	Hearts