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A proton nuclear magnetic resonance technique is demonstrated for ascertaining the real-time contribution of the hexose monophosphate shunt to glucose metabolism in the intact incubated rabbit lens. This measurement requires incubation of the tissue in medium supplemented with [1-13C]glucose, and depends on the presence of the 13C label in the methyl position of lactate which creates satellite resonances by way of 13C - 1H spin-spin scalar coupling. The assumptions required to make the measurement are presented. For lenses maintained under control conditions, a basal level corresponding to 5% hexose monophosphate shunt activity was determined. An eight-fold increase in activity was observed under conditions known to stimulate the shunt.
J A Willis, and
W F Williams, and
T Schleich
July 1987,
Investigative ophthalmology & visual science,
J A Willis, and
W F Williams, and
T Schleich
September 1981,
Experimental eye research,
J A Willis, and
W F Williams, and
T Schleich
January 1988,
Clinical physiology and biochemistry,
J A Willis, and
W F Williams, and
T Schleich
January 1987,
Biochemical and biophysical research communications,
J A Willis, and
W F Williams, and
T Schleich
September 1994,
Molecular and biochemical parasitology,
J A Willis, and
W F Williams, and
T Schleich
December 1981,
Experientia,
J A Willis, and
W F Williams, and
T Schleich
January 1987,
Ophthalmic research,
J A Willis, and
W F Williams, and
T Schleich
June 1971,
Infection and immunity,
J A Willis, and
W F Williams, and
T Schleich
December 1968,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
J A Willis, and
W F Williams, and
T Schleich
January 2001,
Biochemistry. Biokhimiia,
