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Ochratoxin A is a nephrotoxin produced by certain species of Aspergillus and Penicillium. We have found previously that renal but not hepatic P-enolpyruvate carboxykinase, and the mRNA for this enzyme, are rapidly decreased in rats and swine fed 0.1 to 1 mg/kg body weight for a few days. In the present study, we isolated kidney mRNA from rats fed ochratoxin A for 2-5 days. By screening a rat kidney cDNA library with [32P]RNA, we have identified several renal mRNAs whose concentration is changed within 2 days by the toxin. The transcription rate of each mRNA was measured in nuclei isolated from kidneys of rats fed ochratoxin A. The incorporation of [32P]UMP into P-enolpyruvate carboxykinase mRNA and the synthesis of other RNAs were not affected. Therefore, the toxin changes mRNA abundance at the post-transcriptional level.
H Meisner, and
L Polsinelli
July 2000,
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H Meisner, and
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H Meisner, and
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H Meisner, and
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H Meisner, and
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H Meisner, and
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H Meisner, and
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H Meisner, and
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H Meisner, and
L Polsinelli
January 1974,
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H Meisner, and
L Polsinelli
March 1990,
Proceedings of the National Academy of Sciences of the United States of America,
