Biomaterial Database

Infectious RNA transcripts from cloned cDNAs of cucumber mosaic viral satellites. 1986

C W Collmer, and J M Kaper

Complete cDNA copies of two variants of CARNA 5, the satellite of cucumber mosaic virus, have been cloned in the transcription vector pPM1. These two naturally-occurring CARNA 5s are capable and incapable, respectively, of inducing a lethal necrotic disease of tomato upon coinoculation with the genomic RNAs 1, 2, and 3 of cucumber mosaic virus. Uncapped transcripts synthesized in vitro from the two linearized, recombinant plasmids are infectious and each induces the appropriate symptomatology upon coinfection with cucumber mosaic viral RNAs on tomato plants. Progeny CARNA 5s isolated from such infected plants correspond to their natural CARNA 5 counterparts.

UI	MeSH Term	Description	Entries
D009029	Mosaic Viruses	Viruses which produce a mottled appearance of the leaves of plants.	Mosaic Virus,Virus, Mosaic,Viruses, Mosaic
D010935	Plant Diseases	Diseases of plants.	Disease, Plant,Diseases, Plant,Plant Disease
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004276	DNA, Satellite	Highly repetitive DNA sequences found in HETEROCHROMATIN, mainly near centromeres. They are composed of simple sequences (very short) (see MINISATELLITE REPEATS) repeated in tandem many times to form large blocks of sequence. Additionally, following the accumulation of mutations, these blocks of repeats have been repeated in tandem themselves. The degree of repetition is on the order of 1000 to 10 million at each locus. Loci are few, usually one or two per chromosome. They were called satellites since in density gradients, they often sediment as distinct, satellite bands separate from the bulk of genomic DNA owing to a distinct BASE COMPOSITION.	Satellite DNA,Satellite I DNA,DNA, Satellite I,DNAs, Satellite,DNAs, Satellite I,I DNA, Satellite,I DNAs, Satellite,Satellite DNAs,Satellite I DNAs
D012367	RNA, Viral	Ribonucleic acid that makes up the genetic material of viruses.	Viral RNA