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Examination of metal ion-dependent effects on the electrophoretic mobility of bovine prothrombin and fragment 1 provides a useful and sensitive method for investigation of conformational processes in these proteins. Utilization of this method reveals a conformational change in bovine prothrombin and fragment 1 which occurs at low metal ion concentrations. Equilibrium dialysis studies indicate that the metal ion-induced shape change occurs concomitant with binding of a single calcium ion/molecule of prothrombin or fragment 1. Mixed metal electrophoretic mobility studies with Mg2+ and Ca2+ have demonstrated the "synergistic" effect for fragment 1 observed by others. Mixed metal equilibrium dialysis has provided experimental support for this observation and allows us to conclude that two tight Ca2+ sites are not affected by low Mg2+ concentrations and that the third Ca2+ site is also a tight site for Mg2+. Thus, at low Mg2+ concentrations and upon the addition of Ca2+, there are effectively three tight sites; consequently more Ca2+ will bind to the protein at lower total Ca2+ ion concentrations.
D W Deerfield, and
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S Wells, and
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K A Koehler, and
L G Pedersen, and
R G Hiskey
May 1985,
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D W Deerfield, and
P Berkowitz, and
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K A Koehler, and
L G Pedersen, and
R G Hiskey
December 1979,
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D W Deerfield, and
P Berkowitz, and
D L Olson, and
S Wells, and
R A Hoke, and
K A Koehler, and
L G Pedersen, and
R G Hiskey
September 1980,
The Journal of biological chemistry,
D W Deerfield, and
P Berkowitz, and
D L Olson, and
S Wells, and
R A Hoke, and
K A Koehler, and
L G Pedersen, and
R G Hiskey
March 1979,
The Biochemical journal,
D W Deerfield, and
P Berkowitz, and
D L Olson, and
S Wells, and
R A Hoke, and
K A Koehler, and
L G Pedersen, and
R G Hiskey
September 1985,
The Journal of biological chemistry,
D W Deerfield, and
P Berkowitz, and
D L Olson, and
S Wells, and
R A Hoke, and
K A Koehler, and
L G Pedersen, and
R G Hiskey
January 1981,
Biochemistry,
D W Deerfield, and
P Berkowitz, and
D L Olson, and
S Wells, and
R A Hoke, and
K A Koehler, and
L G Pedersen, and
R G Hiskey
September 1992,
Biochemistry,
D W Deerfield, and
P Berkowitz, and
D L Olson, and
S Wells, and
R A Hoke, and
K A Koehler, and
L G Pedersen, and
R G Hiskey
February 1985,
Thrombosis and haemostasis,
D W Deerfield, and
P Berkowitz, and
D L Olson, and
S Wells, and
R A Hoke, and
K A Koehler, and
L G Pedersen, and
R G Hiskey
August 1981,
The Journal of biological chemistry,
D W Deerfield, and
P Berkowitz, and
D L Olson, and
S Wells, and
R A Hoke, and
K A Koehler, and
L G Pedersen, and
R G Hiskey
March 1992,
The Journal of biological chemistry,
