Because of the absence of phosphorescence, the Stern-Volmer constant (KSV) of the photosensitizer is hard to determine accurately. Although the delayed fluorescence and correlated fluorescence methods have been proposed to determine KSV, the weak signal detection and non-uniform excitation enlarged the measurement error. In this work, a method was proposed to accurately determine KSV by oxygen-dependent consumption of 1,3-diphenylisobenzofuran. The consumption time (δ), as a measurable quantity, is introduced and could be obtained by the absorption spectrum with a high signal-to-noise ratio. Analytically, δ is linearly related to the inverse of oxygen content, and the ratio of the intercept to the slope equals KSV. Experimentally, rose Bengal was selected to perform this determination; the KSV is measured to be 43(1) kPa-1, and the error is reduced by 1 order of magnitude. In addition, metalloporphyrin was used to verify this method.