This is a descriptive study that was conducted to examine the effect of work-life balance on job stress and fatigue in taxi drivers. The study included 150 taxi drivers residing in Ankara. As a data collection tool, we used Work-Life Balance, Chalder Fatigue, and Work Stress Scales. We used the Mann-Whitney U test and multiple linear regression analysis. The model established after multiple linear regression analysis was statistically significant ( F [5.142] = 5.442, P < 0.001). A 1-unit increase in the work-life balance variable caused a 2.885-unit decrease in the work stress score and a 0.143-unit decrease in the fatigue score. We can suggest that taxi drivers who have a work-life balance will be able to participate in social activities in their daily lives, and leisure time activities with family and friends can help motivate individuals more and increase energy.