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A method is described for the preparation of rat pulmonary surfactant, radiolabelled specifically in the phosphatidylcholine species, which may be used for degradative studies of the lipoprotein complex. Intravenously administered [methyl-14C]choline chloride is maximally incorporated into alveolar surface surfactant 8 h after injection, and more than 97% of this radiolabel is present in the phosphatidylcholine fraction of the surfactant and, of this, 75% is associated with the dipalmitoyl phosphatidylcholine species. Electron microscopy indicates that the isolated surfactant has a similar physical form to that found at the alveolar surface. The mineral alpha-quartz can be used to increase the yield of surfactant lavaged from the lung surface, but the complex isolated from rats treated in this manner has a low specific radioactivity (less than 1000 d.p.m./mg) compared with that prepared from control animals (22860 d.p.m./mg).
R W Lewis, and
J L Harwood, and
R J Richards
February 1994,
Analytical biochemistry,
R W Lewis, and
J L Harwood, and
R J Richards
January 1978,
Journal of immunological methods,
R W Lewis, and
J L Harwood, and
R J Richards
November 1953,
Biochimica et biophysica acta,
R W Lewis, and
J L Harwood, and
R J Richards
March 1978,
Gigiena truda i professional'nye zabolevaniia,
R W Lewis, and
J L Harwood, and
R J Richards
September 1972,
Mutation research,
R W Lewis, and
J L Harwood, and
R J Richards
August 1918,
Science (New York, N.Y.),
R W Lewis, and
J L Harwood, and
R J Richards
March 1971,
Archives of internal medicine,
R W Lewis, and
J L Harwood, and
R J Richards
January 1978,
Laboratornoe delo,
R W Lewis, and
J L Harwood, and
R J Richards
July 1966,
Thrombosis et diathesis haemorrhagica,
R W Lewis, and
J L Harwood, and
R J Richards
June 1967,
The Anatomical record,
