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A radioimmunoassay for the determination of 6-keto-PGF1 alpha (stable metabolite of Prostacyclin) in urine using a specific antiserum, is described. The antibody used, showed no significant cross-reactivity with other PGS. (3H)-6-keto-PGF1 alpha has been used as tracer and a 30% Hydroxyapatite suspension was utilized for the separation of bound and free fractions. Standard curve ranges between 2 and 1000 pg thus allowing measurements in urine. Aliquots of 0.2 to 1 ml of 24 hours urinary samples were submitted to a preextraction step with Hexane in order to eliminate neutral fats. After acidifying with 0.1 N HCl to pH = 3.5-4, an Ethyl Ether extraction step was performed showing a recovery of 66%. Repeated analysis of a rat urine pool, showed 7.7 intraassay and 9.8 interassay variation coefficients. Values obtained amounted to 38.3 +/- 4.6 ng/24 h in normal rats and 133 +/- 32 ng/24 h in 5% NaCl loaded rats (p less than 0.001). The values obtained in healthy men were 508 +/- 94 ng/24 h and in women 375 +/- 136 (p less than 0.05).
V Lahera, and
F Durán, and
V Cachofeiro, and
F J del Cañizo, and
F J Rodríguez, and
J A Tresguerres
August 1995,
Prostaglandins,
V Lahera, and
F Durán, and
V Cachofeiro, and
F J del Cañizo, and
F J Rodríguez, and
J A Tresguerres
December 1987,
Zhonghua nei ke za zhi,
V Lahera, and
F Durán, and
V Cachofeiro, and
F J del Cañizo, and
F J Rodríguez, and
J A Tresguerres
January 1982,
Methods in enzymology,
V Lahera, and
F Durán, and
V Cachofeiro, and
F J del Cañizo, and
F J Rodríguez, and
J A Tresguerres
August 1986,
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
V Lahera, and
F Durán, and
V Cachofeiro, and
F J del Cañizo, and
F J Rodríguez, and
J A Tresguerres
March 1992,
Thrombosis research,
V Lahera, and
F Durán, and
V Cachofeiro, and
F J del Cañizo, and
F J Rodríguez, and
J A Tresguerres
October 1988,
Prostaglandins,
V Lahera, and
F Durán, and
V Cachofeiro, and
F J del Cañizo, and
F J Rodríguez, and
J A Tresguerres
December 1987,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
V Lahera, and
F Durán, and
V Cachofeiro, and
F J del Cañizo, and
F J Rodríguez, and
J A Tresguerres
March 1986,
Prostaglandins,
V Lahera, and
F Durán, and
V Cachofeiro, and
F J del Cañizo, and
F J Rodríguez, and
J A Tresguerres
December 1998,
Yao xue xue bao = Acta pharmaceutica Sinica,
V Lahera, and
F Durán, and
V Cachofeiro, and
F J del Cañizo, and
F J Rodríguez, and
J A Tresguerres
January 1993,
Brain & development,
