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The technique of frequency-domain fluorometry has been extended to 2000 MHz using the harmonic content of a picosecond laser source and a microchannel plate photomultiplier tube. This new instrument was used to resolve complex subnanosecond intensity and anisotropy decays of the tyrosyl emission of oxytocin. The intensity decay was found to contain at least three exponential components, 80, 359 and 927 ps. The anisotropy analysis revealed a 29 ps torsional motion of the tyrosine residue as well as a 454 ps overall rotational correlation time. The time resolution of this method should permit the comparison of experimental results with theoretical models for motions of proteins.
J R Lakowicz, and
G Laczko, and
I Gryczynski
January 1988,
Photochemistry and photobiology,
J R Lakowicz, and
G Laczko, and
I Gryczynski
May 1988,
The Journal of biological chemistry,
J R Lakowicz, and
G Laczko, and
I Gryczynski
August 1987,
Chemical physics letters,
J R Lakowicz, and
G Laczko, and
I Gryczynski
February 1987,
Analytical biochemistry,
J R Lakowicz, and
G Laczko, and
I Gryczynski
January 1991,
European biophysics journal : EBJ,
J R Lakowicz, and
G Laczko, and
I Gryczynski
October 1988,
Biophysical chemistry,
J R Lakowicz, and
G Laczko, and
I Gryczynski
September 1987,
Applied optics,
J R Lakowicz, and
G Laczko, and
I Gryczynski
December 1996,
Journal of fluorescence,
J R Lakowicz, and
G Laczko, and
I Gryczynski
September 1992,
Biophysical chemistry,
J R Lakowicz, and
G Laczko, and
I Gryczynski
September 1994,
The Journal of physical chemistry,
