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We produced three batches of a human-serum-based enzyme reference material (ERM) enriched with human aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2), creatine kinase (EC 2.7.3.2), and lactate dehydrogenase (EC 1.1.1.27). The added enzymes were not exhaustively purified; thus the final ERMs contained some enzymes as contaminants, of which only glutamate dehydrogenase activity might interfere. The stability during storage and after reconstitution was good. The commutability of the four enzymes in the three ERM batches was also good, except when German or Scandinavian methods for aminotransferases were involved. The temperature-conversion factors for the ERMs were equivalent to those for patients' sera. Reactivation after reconstitution was complete within 5 min and was independent of the temperature of the reconstitution fluid. We believe that these secondary ERMs will aid in the transfer of accuracy between well-defined reference methods and daily working methods so that clinical enzymology results will become more comparable from laboratory to laboratory.
J C Koedam, and
G M Steentjes, and
S Buitenhuis, and
E Schmidt, and
R Klauke
November 1987,
Clinical chemistry,
J C Koedam, and
G M Steentjes, and
S Buitenhuis, and
E Schmidt, and
R Klauke
November 1987,
Clinical chemistry,
J C Koedam, and
G M Steentjes, and
S Buitenhuis, and
E Schmidt, and
R Klauke
April 2001,
Clinica chimica acta; international journal of clinical chemistry,
J C Koedam, and
G M Steentjes, and
S Buitenhuis, and
E Schmidt, and
R Klauke
January 1967,
Progress in immunobiological standardization,
J C Koedam, and
G M Steentjes, and
S Buitenhuis, and
E Schmidt, and
R Klauke
January 1992,
Food additives and contaminants,
J C Koedam, and
G M Steentjes, and
S Buitenhuis, and
E Schmidt, and
R Klauke
September 1979,
Clinical chemistry,
J C Koedam, and
G M Steentjes, and
S Buitenhuis, and
E Schmidt, and
R Klauke
January 1991,
Food additives and contaminants,
J C Koedam, and
G M Steentjes, and
S Buitenhuis, and
E Schmidt, and
R Klauke
January 2004,
Journal of AOAC International,
J C Koedam, and
G M Steentjes, and
S Buitenhuis, and
E Schmidt, and
R Klauke
January 1987,
The Analyst,
J C Koedam, and
G M Steentjes, and
S Buitenhuis, and
E Schmidt, and
R Klauke
February 1994,
Journal of the International Federation of Clinical Chemistry,
