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The microsomes of placenta and uterus from pregnant rabbits have been found to catalyze the omega-hydroxylation of PGE1, PGE2, PGF2 alpha, and PGA1 as well as the omega- and (omega-1)-hydroxylation of palmitate and myristate in the presence of NADPH. These activities were greatly inhibited by carbon monoxide, indicating the involvement of cytochrome P-450. The apparent Km for PGE1 was 2.38 microM and 2.1 microM with the placental and uterus microsomes, respectively. Cytochrome P-450 has been solubilized with 1% cholate from the placental microsomes, and partially purified by chromatography on 6-amino-n-hexyl Sepharose 4B, DEAE-Sephadex A-50 and hydroxylapatite columns. The partially purified cytochrome P-450 efficiently catalyzed the omega-hydroxylation of various prostaglandins such as PGE1, PGE2, PGF2 alpha, PGD2, and PGA1 in a reconstituted system containing NADPH-cytochrome P-450 reductase, cytochrome b5, and phosphatidylcholine. The reconstituted system also hydroxylated palmitate and myristate at the omega- and (omega-1)-position, but could not hydroxylate laurate. These catalytic properties resemble those of a new form of cytochrome P-450 highly purified from the lung microsomes of progesterone-treated rabbits (Yamamoto, S., Kusunose, E., Ogita, K., Kaku, M., Ichihara, K., and Kusunose, M. (1984) J. Biochem. 96, 593-603). This type of cytochrome P-450, viz., cytochrome P-450 with high prostaglandin omega-hydroxylase activity may play a role in the regulation of prostaglandin levels in pregnancy.
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E Kusunose, and
S Matsubara, and
K Ichihara, and
M Kusunose
January 1989,
Advances in prostaglandin, thromboxane, and leukotriene research,
S Yamamoto, and
E Kusunose, and
S Matsubara, and
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M Kusunose
January 1990,
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S Yamamoto, and
E Kusunose, and
S Matsubara, and
K Ichihara, and
M Kusunose
August 1989,
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S Yamamoto, and
E Kusunose, and
S Matsubara, and
K Ichihara, and
M Kusunose
December 1984,
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S Yamamoto, and
E Kusunose, and
S Matsubara, and
K Ichihara, and
M Kusunose
November 1987,
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S Yamamoto, and
E Kusunose, and
S Matsubara, and
K Ichihara, and
M Kusunose
February 1990,
Journal of biochemistry,
S Yamamoto, and
E Kusunose, and
S Matsubara, and
K Ichihara, and
M Kusunose
August 1990,
Journal of biochemistry,
S Yamamoto, and
E Kusunose, and
S Matsubara, and
K Ichihara, and
M Kusunose
August 1968,
Biochimica et biophysica acta,
S Yamamoto, and
E Kusunose, and
S Matsubara, and
K Ichihara, and
M Kusunose
September 1989,
Journal of biochemistry,
S Yamamoto, and
E Kusunose, and
S Matsubara, and
K Ichihara, and
M Kusunose
January 1988,
Journal of biochemistry,
