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We have determined the complete sequence of apolipoprotein (apo) B-100 cDNA. It is 14.1 kilobases in length and codes for a 4563-amino acid protein, including a 27-amino acid signal peptide and a 4536-amino acid mature protein. Further, we identified 2366 residues of apoB-100 by direct sequence analysis of apoB-100 tryptic peptides. The mature peptide is characterized by high hydrophobicity (0.916 kcal/residue) and predicted beta-sheet content (21%). Dot matrix analysis revealed the presence of many long internal repeats in apoB-100. The mature peptide contains 25 cysteine residues, 12 of which are in the N-terminal 500 residues. Twenty potential N-linked glycosylation sites were identified, of which 13 were proven to be glycosylated, and 4 were found not to be glycosylated by direct analysis of tryptic peptides. Our findings on apoB structure provide a basis for future experimentation on the role of apoB-100-containing lipoproteins in atherosclerosis.
S H Chen, and
C Y Yang, and
P F Chen, and
D Setzer, and
M Tanimura, and
W H Li, and
A M Gotto, and
L Chan
November 1986,
Proceedings of the National Academy of Sciences of the United States of America,
S H Chen, and
C Y Yang, and
P F Chen, and
D Setzer, and
M Tanimura, and
W H Li, and
A M Gotto, and
L Chan
September 1986,
Nucleic acids research,
S H Chen, and
C Y Yang, and
P F Chen, and
D Setzer, and
M Tanimura, and
W H Li, and
A M Gotto, and
L Chan
April 1982,
The Journal of biological chemistry,
S H Chen, and
C Y Yang, and
P F Chen, and
D Setzer, and
M Tanimura, and
W H Li, and
A M Gotto, and
L Chan
May 1984,
FEBS letters,
S H Chen, and
C Y Yang, and
P F Chen, and
D Setzer, and
M Tanimura, and
W H Li, and
A M Gotto, and
L Chan
August 1986,
Nucleic acids research,
S H Chen, and
C Y Yang, and
P F Chen, and
D Setzer, and
M Tanimura, and
W H Li, and
A M Gotto, and
L Chan
May 1984,
Proceedings of the National Academy of Sciences of the United States of America,
S H Chen, and
C Y Yang, and
P F Chen, and
D Setzer, and
M Tanimura, and
W H Li, and
A M Gotto, and
L Chan
October 1984,
The Journal of biological chemistry,
S H Chen, and
C Y Yang, and
P F Chen, and
D Setzer, and
M Tanimura, and
W H Li, and
A M Gotto, and
L Chan
April 1987,
Biochemical Society transactions,
S H Chen, and
C Y Yang, and
P F Chen, and
D Setzer, and
M Tanimura, and
W H Li, and
A M Gotto, and
L Chan
July 1987,
Proceedings of the National Academy of Sciences of the United States of America,
S H Chen, and
C Y Yang, and
P F Chen, and
D Setzer, and
M Tanimura, and
W H Li, and
A M Gotto, and
L Chan
July 1987,
Seminars in thrombosis and hemostasis,
