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Characterization of quinolones resistant Clostridium perfringens toxinotype D. 2023

- Madeeha Tariq, and - Aftab Ahmad Anjum, and - Tehreem Ali, and - Mian Muhammad Khubaib Sattar, and - Rabia Manzoor
Institute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.

Clostridium perfringens toxins play role in causing pulpy kidney disease poisoning as well enterotoxaemia. To combat antimicrobial resistance: curbing use, regulations and execution to antimicrobial usage in food along with withdrawal period is necessary. Aim of study was to optimize the toxins production by indigenously characterized C. perfringens type D isolates (n=03) under various physicochemical parameters, a lead towards local vaccine production in Pakistan. Indigenous isolates were characterized on the basis of 16S rRNA and MW349974.1, MW341428.1, MW332258.1 accession numbers were received from NCBI GenBank. Isolates were identified as toxinotype D through PCR toxinotyping. Quinolones antibiotic susceptibility testing revealed that isolates observed susceptible to enrofloxacin and resistant to ciprofloxacillin and ofloxacillin. Optimization of toxins production was determined under the influence of physical and chemical parameters. Alpha and epsilon toxin production in reinforced clostridial medium (RCM) broth was observed higher at 37°C after 24h incubation by MW332258.1. Under the influence of 0.2% glucose and 0.3% tween 80 supplementation in RCM, greater production of alpha and epsilon toxin units was observed by MW332258.1. Under optimized physicochemical parameters, maximum toxins units were observed; MW332258.1 isolate is excellent candidate could be used to produce maximum toxin units for vaccine production at industrial scale.

UI MeSH Term Description Entries
D003016 Clostridium perfringens The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins. Clostridium welchii
D005947 Glucose A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement. Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D012336 RNA, Ribosomal, 16S Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis. 16S Ribosomal RNA,16S rRNA,RNA, 16S Ribosomal,Ribosomal RNA, 16S,rRNA, 16S
D014612 Vaccines Suspensions of killed or attenuated microorganisms (bacteria, viruses, fungi, protozoa), antigenic proteins, synthetic constructs, or other bio-molecular derivatives, administered for the prevention, amelioration, or treatment of infectious and other diseases. Vaccine
D015363 Quinolones A group of derivatives of naphthyridine carboxylic acid, quinoline carboxylic acid, or NALIDIXIC ACID. Ketoquinoline,Ketoquinolines,Oxoquinoline,Oxoquinolines,Quinolinone,Quinolinones,Quinolone

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