Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle. 2023

Naglaa Ebrahim Aly, and Mohamed Hassan Atwa, and Amany Mohamed Abbas, and Diana Mohamed Abulmagd, and Zeinab Taha Salem, and Taradi Abdelfattah Sayed
Department of Pet Animal Vaccine, Agriculture Research Center (ARC), Veterinary Serum and Vaccine Research Institute, Abasia, Cairo, Egypt.

The target of vaccination is to encourage a strong, covering and long-lasting immune response against antigens. For achieving these objectives; effective adjuvant and new vaccine strategies are demanded to make the vaccine sufficiently immunogenic to instigate a powerful immune response. This study was completed for elaboration and evaluation of freeze-dried combined vaccine against both Rift Valley fever (RVF) and bovine ephemeral fever (BEF) viruses using different stabilizers. Three formulae were prepared from such vaccine including: formula (1): stabilized with a mixture of 5% Lactalbumin Hydrolysate and 2.5% sucrose, formula (2): stabilized with a mixture of 50% the previous stabilizer and 50% of 1% Carbopol and formula (3): stabilized with 1% Carbopol solution. Samples of the three vaccine formulae were reconstituted on the time of experimental animal vaccination using saponin diluent which acts as an adjuvant for both RVFv and BEFv and as an inactivator BEF virus. The ratio between both viruses in all vaccine formulae was 1:1. All vaccine batches were proved to be free of any foreign contaminants and unharmed for experimentally vaccinated animals. Each of the three groups of calves was vaccinated S/C with 2 ml of a reconstituted vaccine formula and their immune response was evaluated using serum neutralization test. The gained results revealed that the prepared combined freeze-dried vaccine with Carbopol elicited a better humoral immune response than the other two vaccine formulae. It could be recommended to use Carbopol as a stabilizer for the preparation of the aimed vaccine.

UI MeSH Term Description Entries
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002418 Cattle Diseases Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus. Bovine Diseases,Bovine Disease,Cattle Disease,Disease, Bovine,Disease, Cattle,Diseases, Bovine,Diseases, Cattle
D004810 Ephemeral Fever An Ephemerovirus infection of cattle caused by bovine ephemeral fever virus (EPHEMERAL FEVER VIRUS, BOVINE). It is characterized by respiratory symptoms, increased oropharyngeal secretions and lacrimation, joint pains, tremor, and stiffness. Three-Day Sickness,Fever, Ephemeral,Sickness, Three-Day,Three Day Sickness
D000277 Adjuvants, Pharmaceutic Agents that aid or increase the action of the principle drug (DRUG SYNERGISM) or that affect the absorption, mechanism of action, metabolism, or excretion of the primary drug (PHARMACOKINETICS) in such a way as to enhance its effects. Adjuvant, Pharmaceutic,Adjuvant, Pharmaceutical,Adjuvants, Pharmaceutical,Pharmaceutic Adjuvant,Pharmaceutic Adjuvants,Pharmaceutical Adjuvant,Pharmaceutical Adjuvants
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012295 Rift Valley Fever An acute infection caused by the RIFT VALLEY FEVER VIRUS, an RNA arthropod-borne virus, affecting domestic animals and humans. In animals, symptoms include HEPATITIS; abortion (ABORTION, VETERINARY); and DEATH. In humans, symptoms range from those of a flu-like disease to hemorrhagic fever, ENCEPHALITIS, or BLINDNESS. Fever, Rift Valley
D014611 Vaccination Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis. Immunization, Active,Active Immunization,Active Immunizations,Immunizations, Active,Vaccinations
D017778 Vaccines, Combined Two or more vaccines in a single dosage form. Bivalent Vaccine,Bivalent Vaccines,Combined Vaccine,Combined Vaccines,Decavalent Vaccine,Decavalent Vaccines,Heptavalent Vaccine,Heptavalent Vaccines,Hexavalent Vaccine,Hexavalent Vaccines,Multivalent Vaccine,Multivalent Vaccines,Nonavalent Vaccine,Nonavalent Vaccines,Octavalent Vaccine,Octavalent Vaccines,Pentavalent Vaccine,Pentavalent Vaccines,Polyvalent Vaccine,Polyvalent Vaccines,Quadrivalent Vaccine,Quadrivalent Vaccines,Tetravalent Vaccine,Tetravalent Vaccines,Vaccines, Multivalent,Vaccines, Polyvalent,Vaccines, Combination,Combination Vaccines,Vaccine, Bivalent,Vaccine, Combined,Vaccine, Decavalent,Vaccine, Heptavalent,Vaccine, Hexavalent,Vaccine, Multivalent,Vaccine, Nonavalent,Vaccine, Octavalent,Vaccine, Pentavalent,Vaccine, Polyvalent,Vaccine, Quadrivalent,Vaccine, Tetravalent,Vaccines, Bivalent,Vaccines, Decavalent,Vaccines, Heptavalent,Vaccines, Hexavalent,Vaccines, Nonavalent,Vaccines, Octavalent,Vaccines, Pentavalent,Vaccines, Quadrivalent,Vaccines, Tetravalent

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