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The presence of carbohydrates in homogeneous preparations of human liver acid alpha-glucosidase has been established and the carbohydrate content of the enzyme determined. The enzyme was purified with the specific purpose of removing all low-molecular-weight carbohydrates. It was specifically adsorbed on Concanavalin A-Sepharose, eluted with methyl-alpha-D-mannopyranoside and gave a positive reaction with the phenol-sulphuric acid reagent. These facts taken together provide evidence that the enzyme studied is a glycoprotein. The analysis of the carbohydrate content of human liver acid alpha-glucosidase showed that there were 8.3 glucosamine, 13.2 mannose and possibly 3--4 glucose residues per molecule of the enzyme with a molecular weight of 98,000.
D M Belen'ky, and
V I Mikhajlov, and
E L Rosenfeld
May 1975,
Clinica chimica acta; international journal of clinical chemistry,
D M Belen'ky, and
V I Mikhajlov, and
E L Rosenfeld
January 1975,
Biokhimiia (Moscow, Russia),
D M Belen'ky, and
V I Mikhajlov, and
E L Rosenfeld
January 1978,
Doklady Akademii nauk SSSR,
D M Belen'ky, and
V I Mikhajlov, and
E L Rosenfeld
August 1971,
Experientia,
D M Belen'ky, and
V I Mikhajlov, and
E L Rosenfeld
January 1978,
Archives of biochemistry and biophysics,
D M Belen'ky, and
V I Mikhajlov, and
E L Rosenfeld
May 1977,
Biochimica et biophysica acta,
D M Belen'ky, and
V I Mikhajlov, and
E L Rosenfeld
January 1983,
Enzyme,
D M Belen'ky, and
V I Mikhajlov, and
E L Rosenfeld
January 1962,
Biokhimiia (Moscow, Russia),
D M Belen'ky, and
V I Mikhajlov, and
E L Rosenfeld
July 1982,
Biokhimiia (Moscow, Russia),
D M Belen'ky, and
V I Mikhajlov, and
E L Rosenfeld
January 1987,
Biochimica et biophysica acta,
