Biomaterial Database

Electrical measurement of chloride secretion in the perfused human jejunum: effects of theophylline and prostaglandin E1. 1986

H Masukawa, and M Kaneko, and T Takahashi, and M Ishikawa, and Y Suzuki

The effects of intraluminal theophylline or prostaglandin E1 (PGE1) on transepithelial potential difference (PD) were examined in the human jejunum in vivo with a quadruple lumen perfusion tube. To measure transepithelial PDs, we placed one of the Ag/AgCl electrodes in the jejunal lumen and the other in the subcutaneous region of the left forearm of the subjects. Infusion of theophylline or PGE1 into the jejunal lumen produced a change in the PD, the lumen being more negatively charged. The magnitude of the change in PD for 2.4 X 10(-3) M theophylline was 3.3 +/- 0.48 mV (mean +/- S.E., n = 6) and that for 10(-6) M PGE1 was 5.0 +/- 0.60 mV (n = 6). In addition, the change in the PD induced by simultaneous infusion of both 2.4 X 10(-3) M theophylline and 10(-7) M PGE1 was about twice as large as the sum of those induced by individual infusion of the same concentration of theophylline or PGE1. We concluded that these PD changes occurred primarily by stimulation of electrogenic Cl secretion in the jejunum. Further exploitation of this method to determine PD might be useful for clinical investigation of the intestinal secretion.

UI	MeSH Term	Description	Entries
D007583	Jejunum	The middle portion of the SMALL INTESTINE, between DUODENUM and ILEUM. It represents about 2/5 of the remaining portion of the small intestine below duodenum.	Jejunums
D008297	Male		Males
D008564	Membrane Potentials	The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).	Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D010477	Perfusion	Treatment process involving the injection of fluid into an organ or tissue.	Perfusions
D002712	Chlorides	Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.	Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D000527	Alprostadil	A potent vasodilator agent that increases peripheral blood flow.	PGE1,Prostaglandin E1,Caverject,Edex,Lipo-PGE1,Minprog,Muse,PGE1alpha,Prostaglandin E1alpha,Prostavasin,Prostin VR,Prostine VR,Sugiran,Vasaprostan,Viridal,Lipo PGE1